Technical Data
Brain Derived Neurotrophic Factor (BDNF) BioAssay™ ELISA Kit
Kits and Assays Storage: 4°CShipping: Blue Ice
Brain Derived Neurotrophic Factor (BDNF) kit is a sandwich enzyme immunoassay (EIA), which measures BDNF. Kit is designed to measure the amount of BDNF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from human and rat. With the BDNF assay system, rabbit polyclonal antibodies generated against human BDNF are coated onto a microplate and are used to capture BDNF from a sample. BDNF specific, biotin conjugated, mouse monoclonal antibodies detect the captured BDNF. After addition of streptavidin, enzyme, substrate and stop solution the amount of BDNF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and BDNF concentration, i.e., the higher the OD the higher the BDNF concentration in the sample.


Range of Detection:

No significant crossreactivity with NGF, NT4/5 or NT3

Intra-assay Variation:
± 3.7% (125pg/ml)

Inter-assay Variation:
± 8.5% (125pg/ml)

Kit Components:
There are enough reagents included in this kit for two 96-well immuno-assay plates.
B2700-08A: BDNF ELISA Plates, 2x1 plate Pre-coated with Rabbit anti-Human BDNF Polyclonal Antibody 96-Well Microtiter plates.
B2700-08B: Wash Buffer, 10X, 1x100ml
B2700-08C: Standard/Sample Diluent, 1x60ml
*B2700-08D: BDNF Standard (Recombinant Human), 2x1 vials (Lyophilized).
B2700-08E: Mab (Biotin), 1x25ul
B2700-08F: Streptavidin (HRP), 1x50ul
B2700-08G: TMB/E Solution, 2x10ml
B2700-08H: Stop Solution (HCl), 1x22ml

Storage and Stability:
Store at 4°C. Do not freeze. Stable for 6 months. For maximum recovery of product, centrifuge the original vial prior to removing the cap.
*B2700-08D: BDNF Standard, Store at 4°C for up to 30 days (liquid).
Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.
1. Laske, C., et al. (2007) Increased BDNF serum concentration in fibromyalgia with or without depression or antidepressants. Journal of Psychiatric Research. 41:600-605. 2. Tramontina, J., et al. (2007) Val66met polymorphism and serum brain-derived neurotrophic factor levels in bipolar disorder. Molecular Psychiatry. 12:230- 231. 3. Neumeister, A., et al., (2005) Effects of Tryptophan Depletion on Serum Levels of Brain-Derived Neurotrophic Factor in Unmedicated Patients with Remitted Depression and Healthy Subjects. Am. J. Psychiatry. 162:805-807.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.