Technical Data
Brain Natriuretic Peptide (BNP)
Brain natriuretic peptide is a 32 amino acid polypeptide secreted by the ventricles of the heart in response to excessive stretching of myocytes in the ventricles. At the time of release, a co-secreted 76 amino acid N-terminal fragment (NT-proBNP) is also released with BNP. BNP binds to and activates NPRA in a similar fashion to atrial natriuretic peptide (ANP) but with 10-fold lower affinity.

Suitable for use in Westen Blot Other applications have not been tested.

Recommended Dilution:
Western Blot: 0.5ug/ml detects BNP in 10ug rat spleen lysate.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies
I1904-39: IgG, X-Adsorbed (HRP) Pab Gt xRb
I1904-40A: IgG, H&L, X-Adsorbed (HRP) Pab Gt xRb
I1904-46Q: IgG, H&L (HRP) Pab Gt xRb

Rat Spleen Lysate.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceRatRabbit
Synthetic peptide corresponding to rat Brain Natriuretic Peptide.
Purified by affinity chromatography.
Supplied as a liquid in PBS, 0.09% sodium azide.
Recognizes rat Brain Natriuretic Peptide.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.