Technical Data
B2727-01C
BRCC-36 (BRCA1/BRCA2-containing Complex Subunit 36, BRCC36, BRCA1/BRCA2-containing Complex Subunit 3, BRCC3, C6.1A, CXorf53, RP11-143H17.2)
Description:
BRCC36 (BRCA1/BRCA2-containing complex, subunit 36) is a holoenzyme complex containing BRCA1, BRCA2. BRCC36 is an ubiquitin E3 ligase complex that has a role in DNA repair and enhances cellular survival following DNA damage. This protein is also thought to be involved in the cellular response to ionizing radiation and progression through the G2/M checkpoint.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000-1:2000
Optimal dilutions to be determined by the researcher.

Hybridoma:
FO myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG1,k10B2564Affinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Recombinant protein corrosponding to aa1-316 of humunBRCC36 expressed in E. coli (NP_077308).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.1% sodium azide.
Specificity:
Recognizes human BRCC-36
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wang Bin, et al., (2007) Proc Natl Acad Sci USA 104(52):20759-20763 2. Chen Xiaowei, et al., (2006) Cancer Research 66:5039-5046 3. Dong Y, et al., (2003) Mol Cell 12(5):1087-1099