Technical Data
Media
Brilliant Green Agar w/Sulfadiazine (Powder)
B2750-02
Microbiological Grade
Storage RT/4C    Shipping RT
Components shown as g/liter
Agar20.0
Lactose10.0
Sucrose10.0
Pancreatic Digest of Casein5.0
Peptic Digest of Animal Tissue5.0
Sodium Chloride5.0
Yeast Extract3.0
Phenol Red0.08
Sulfadiazine0.08
Brilliant Green0.0125
Total58.1725 g/L
Selective isolation of Salmonella from food, especially egg products.

Description:
Brilliant Green Agar was first described by Kristensen et al. in 1925 and later modified by Kauffman in 1933. It is classified by the USP as a highly selective medium for the recovery of Salmonella species except for tyhphoid and paratyphoid strains. Brilliant Green dye inhibits Gram positive and most Gram negative bacteria. Phenol red serves as a pH indicator for the fermentation of lactose or sucrose in the medium. Osborne and Stokes used 0.1% Sodium Sulfapyridine to enhance the recovery of Salmonella from whole egg and egg yolk. The use of sulfonamides further inhibits Escherichia coli and Proteus sp.

Storage:
Store the sealed bottle containing the dehydrated medium at 2 to 30.0C. Once opened and recapped, place the container in a low humidity environment at the same storage temperature. Protect it from moisture and light. The dehydrated medium should be discarded if it is not free flowing or if the color has changed from the original light greenish beige color.


Directions: 1. Dissolve 58grams per liter of distilled/ deionized (DDI) water, heating with stirring until completely solubilized.

2. Dispense into appropriate containers, loosen caps and autoclave for 15 minutes at 121C (15psi). Storage:Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Microbiological Analysis: Enterococcus faecalis ATCC 29212

Appearance: Light beige with a greenish tint, homogeneous, free flowing powder.

Solubility: Brownish-green, slightty turbid.

pH (25C): 6.90.2


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.