Technical Data
BubR1 (BUB1beta, BUB1B, Bub1A, hBUBR1, MAD3L, MAD3/BUB1-related Protein Kinase, Mitotic Checkpoint Kinase MAD3L, Mitotic Checkpoint Serine/threonine-protein Kinase BUB1 beta, Protein SSK1)
The Mitotic Checkpoint Complex (MCC) , which contains Bub1, Bub1b, Bub3, Mad2 and Cdc20, controls chromosome segregation and monitors kinetochoremicrotubule interactions (1). This complex inhibits the ubiquitin ligase activity of the Anaphase Promoting Complex/ Cyclosome (APC/C) during mitosis to prevent cells with unaligned chromosomes from prematurely entering
anaphase (2). Bub1b and Bub1 kinases are mutated in several types of human malignancies including hematopoietic, colorectal, lung, and breast cancers (3). Biallelic mutations in Bub1b have been found in mosaic variegated aneuploidy syndrome and premature chromatid separation syndrome (4). Bub1b mouse germline knockouts are embryonic lethal with heterozygous animals displaying genetic instability, early aging phenotypes and increased cancer susceptibility (5). Bub3 binds both Bub1 and Bub1b, facilitating their recruitment to kinetochores (6) and is required for functional microtubule-kinetochore interactions (7).

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000 Incubate membrane with diluted antibody in 5% BSA, 1X TBS, 0.1% Tween-20 at 4C with gentle shaking, overnight.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide corresponding to aa sequence surrounding Lys370 of human Bub1b.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, less than 0.02% sodium azide, 50% glycerol.
Recognizes endogenous levels of total Bub1b.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
(1) Fukagawa, T. (2008) Front Biosci 13, 2705-13. (2) Chen, R.H. (2007) J Biomed Sci 14, 475-9.
(3) Dai, W. et al. (2004) Cancer Res 64, 440-5. (4) Kops, G.J. et al. (2005) Nat Rev Cancer 5, 773-85.
(5) Baker, D.J. et al. (2004) Nat Genet 36, 744-9. (6) Taylor, S.S. et al. (1998) J Cell Biol 142, 1-11.
(7) Logarinho, E. et al. (2008) Mol Biol Cell 19, 1798-813.