Technical Data
Bromodeoxyuridine (BrdU)
BrdU is a thymidine analog that, when added to replicateing cells, is incorporated into newly synthesized DNA strands. Evaluating the uptake of BrdU by dividing cells can provide a reliable estimation of cellular prolieration rates. BrdU immunohistochemistry may prove useful in determining proliferative rates for the evaluation of tumors.

Positive Control: Bromodeoxyuridine labeled cells.

Suitable for use in Immunofluorescence, Flow Cytometry, Immunohistochemistry (Fr) (paraffin-embedded sections) and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceMouse
Not determined
BrdU conjugated to BSA.
PBS with 1% BSA containing 0.09% sodium azide
The antibody is specific for DNA in which BrdU has been incorporated. In immunoassays this antibody reacts strongly with free or carrier-protein coupled BrdU but not with other nucleosides. In immuncytochemistry the antibody only recognizes BrdU in denaturated (single stranded) DNA. The BrdU antibody is 100% crossreactive with Iodo-Deoxy-Uridine (IrdU). Therfore, IdU instead of BrdU can be used in studies. Species Crossreactivity: Crossreacts with human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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