Technical Data
B2850-03
Bromodeoxyuridine (BrdU)
Description:
Applications:
Suitable for use in Immunofluorescence microscopy. Also suitable for Detection of bromodeoxyuridine incorporation in tissue and cell culture; 3H-thymidine labeling techniques can be avoided.

Recommended Dilution:
Immunofluorescence microscopy: 1:10; Incubation Time 30 min at RT.

Storage and Stability:
Lyophilized powder may be stored at 4C for short-term only. Reconstitute to nominal volume by adding sterile 40-50% glycerol and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a5F172Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouse
Concentration:
~0.1mg/ml
Immunogen:
Bromodeoxyuridine.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a lyophilized powder in PBS, pH 7.4, 0.5% BSA, 0.09% sodium azide. Reconstitute to a final volume of 1ml.
Specificity:
Specific for bromodesoxyuridine and does not crossreact with thymidine. It is a valuable tool for detection of S-phase (DNA-synthesizing cells) in cell and tissue culture.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Hosihino, T., Nagashima, T., Murovic, J., Levin, E.L., Levin V.A. and Rupp, St.M.: Cell kinetic studies of in situ human brain tumors with bromodeoxyuridine. Cytometrie 6, 627-632 (1985).
Jirikowski G.F., Ramalho-Ortigao F., Kesse K.W. and Bloom F.E.: In situ hybridization of semithin Epon sections with BrdU labelled oligonucleotide probes. Histochemistry 94, 187-190 (1990)