Technical Data
B2850-07
Bromodeoxyuridine (BrdU)
Description:
BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Applications:
Suitable for use in Flow Cytometry, Immuno uorescence and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry (formalin/paraf n): 1:500 for 32 minutes at RT. For staining of formalin- xed tissues, incubate sections in 4N HCl for 30 minutes at RT, followed by digestion with trypsin at 1mg/ml PBS, 10 minutes at 37ēC .
Optimal dilutions to be determined by the researcher.

Positive Control:
Cultured cells grown in prescence of BrdU or tissues from experimental animals injected with BrdU.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG10.N.35Affinity Purified
SizeStorageShippingSourceHost
500ul-20°CBlue IceMouse
Concentration:
~0.2mg/ml
Immunogen:
Bromodeoxyuridine (BrdU) conjugated to BSA. Cellular Localization: Nuclear
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See B2850-07X.
Specificity:
Recognizes Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU conjugated to a protein carrier, as well as free BrdU. Species Crossreactivity: all species
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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