Technical Data
B2850-10B
Bromodeoxyuridine (BrdU)
Description:
Halogenated nucleotides such as the pyrimidine analog bromodeoxyuridine (BrdU) are useful for labeling nascent DNA in living cells and tissues (1,2). BrdU becomes incorporated into replicating DNA in place of thymidine and subsequent immunodetection of BrdU using specific monoclonal antibodies allows labeling of cells in S phase of the cell cycle (3,4). After pulse-labeling cells or tissues with bromodeoxy-uridine, BrdU (Bu20a) Mouse mAb can be used to detect BrdU incorporated into single stranded DNA. Please see our detailed protocol for information regarding the labeling procedure as well as denaturation of double stranded DNA for various immunodetection applications.

Applications:
Suitable for use in Immunohistochemistry (P), Immunofluorescence (IF-IC) and Flow Cytometry
Other applications not tested.

Recommended Dilution:
Immunohistochemistry (P): 1:100 Unmasking buffer: Citrate
Immunofluorescence (IF-IC): 1:1400
Flow Cytometry: 1:800
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG110E10
SizeStorageShippingSourceHost
100ul-20CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
BrdU conjugated to BSA.
Purity:
Form
Supplied in liquid 10mM sodium HEPES pH 7.5, 150mM NaCl, 0.1mg/ml BSA, 50% glycerol and 0.02% sodium azide.
Specificity:
Recognizes human BrdU when incorporated into single stranded DNA. DNA must be denatured for the epitope to be exposed and recognized by the antibody.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Darzynkiewicz, Z. and Juan, G. (2001) Curr Protoc Cytom Chapter 7, Unit 7.7, 2. Leif, R.C. et al. (2004) Cytometry A 58, 45-52, 3. Staszkiewicz, J. et al. (2009) Biochem Biophys Res Commun 378, 539-44, 4. Rothaeusler, K. and Baumgarth, N. (2007) Curr Protoc Cytom Chapter 7, Unit7.31.