Technical Data
B2850-24B
Bromodeoxyuridine (BrdU)
Description:
BrdU is a thymidine analog, incorporated into cell nuclei during DNA synthesis prior to mitosis. Antibody to BrdU is helpful in detecting S-phase cells, providing useful information on the aggressiveness of tumors.

Applications:
Immunohistology (Formalin/paraffin): (Use Ab at 1ug/ml for 30 min at RT) [For staining of formalin-fixed tissues, incubate sections in 4N HCl for 30 minutes at RT followed by digestion with trypsin at 1mg/ml PBS, 10 min at 37C]

Positive Control: Cultured cells grown in presence of BrdU or tissues from experimental animals
injected with BrdU.

Cellular Localization: Nuclear

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Store at -20C or colder. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG15G15Affinity Purified
SizeStorageShippingSourceHost
250ul-20CBlue IceMouse
Concentration:
~0.2mg/ml
Immunogen:
BrdU conjugated to BSA.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA and 0.09% sodium azide. Also available without BSA and azide at 1mg/ml.
Specificity:
Reacts with Bromodeoxyuridine (BrdU) in single stranded DNA (produced by partial denaturation of double stranded DNA), BrdU coupled to a protein carrier, as well as free BrdU. Species Crossreactivity: All species.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Magaud J P, et al. (1989) J Histochem Cytochem, 37:1517-1527.