		Technical Data
B2852-83B	BRD4 (Bromodomain-containing Protein 4, Protein HUNK1, HUNK1)

Description:
Brd4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; longer variant has a C-terminus region with a proline and glutamine-rich domains. Brf4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. Brd4 acts at different stages of cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. Brf4 functions as a cellular target for E2 protein of papillomavirus.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 2-5ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Rabbit

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to aa150-200 of human Brd4.

Purity:

Purified by Protein G affinity Chromatography.

Form

Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.

Specificity:

Recognizes human Brd4. Species Crossreactivity: chimpanzee, mouse, rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Maruyama et.al Mol Cell Biol 22 (18) 6509-6520 (September 2002). 2. Farina et.al Mol Cell Biol 24 (20) 9059-9069 (October 2004). 3. McPhillips et.al J Virol 80 (19) 9530-9543 (October 2006).