Technical Data
B2852-83B
BRD4 (Bromodomain-containing Protein 4, Protein HUNK1, HUNK1)
Description:
Brd4, a ubiquitously expressed nuclear phosphoprotein, is a member of BET protein family. It is a human homolog of mouse MCAP and consists of 2 N-terminal bromodomains, a central ET-domain, and several kinase-like motifs in its N-terminus. It is expressed as 2 isoforms; longer variant has a C-terminus region with a proline and glutamine-rich domains. Brf4 is a chromatin-binding protein whose expression is induced in response to growth stimuli. Brd4 acts at different stages of cell cycle by interacting with proteins including RFC and SPA-1 and regulates cell growth. It also has a role in regulating chromosomal dynamics during mitosis. Brf4 functions as a cellular target for E2 protein of papillomavirus.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilutions:
Western Blot: 2-5ug/ml
Optimal dilutions to be determined by the researcher.

Positve Control:
HeLa cell lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanRabbit
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide corresponding to aa150-200 of human Brd4 (NP_490597).
Purity:
Purified by Protein G affinity Chromatography.
Form
Supplied as a liquid in PBS, 0.05% BSA, 0.05% sodium azide.
Specificity:
Recognizes human Brd4. BRD4 is expressed in two forms, the long form is approximately 1400 amino acids and the short form is approximately 722 amino acids. Species Crossreactivity: chimpanzee, mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Maruyama et.al Mol Cell Biol 22 (18) 6509-6520 (September 2002). 2. Farina et.al Mol Cell Biol 24 (20) 9059-9069 (October 2004). 3. McPhillips et.al J Virol 80 (19) 9530-9543 (October 2006).