Technical Data
BTLA (B- and T-lymphocyte Attenuator, B- and T-lymphocyte-associated Protein, CD272)
B and T lymphocyte attenuator (BTLA) is a 289aa recently identified inhibitory receptor that provides crucial activating or attenuating signals for T cells. It is a third member of co-inhibitory receptors of the CD28 immunoglobulin superfamily with similarities to (CTLA)-4 and (PD)-1 and interacts with a B7 homolog, B7H4. BTLA is constitutively expressed on most CD4+ and CD8+ T cells and its expression progressively decreases upon T cell activation. It remains expressed on Th1 cells but not Th2 cells. BTLA is a unique co-receptor which interacts with the tumor necrosis factor receptor superfamily member Herpes virus entry mediator (HVEM) and this interaction is an important pathway regulating lymphocyte activation and/or homeostasis in the immune response and might also play an important role in the maintenance of T cell tolerance.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 3-5ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human spleen lysate

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceMouseRabbit
Synthetic peptide corresponding to aa1-50 of mouse BTLA.
Purified by Protein G affinity Chromatography.
Supplied as a liquid in PBS, 0.2% gelatin, 0.05% sodium azide.
Recognizes mouse BTLA. Species sequence homology: chicken, canine, human, rat, xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Otsuki, N. et al. Biochem. Biophys. Res. Commun. 344:1121-1127 (2006). 2. Gonzalez, LC. et al. Proc. Natl. Acad. Sci. USA. 102:1116-1121 (2005). 3. Wang, XF. et al.Tissue Antigens. 69:145-153 (2007).