Technical Data
C-1 Inhibitor
C1 Inhibitor is a plasma protein that plays a pivotal role in the regulation of the early stages of the classical complement pathway and the contact activation pathways. It is a protease inhibitor acting as a competitive substrate for its target enzymes. These cleave a 34 amino acid peptide, the remainder of the C1 Inhibitor molecule binding to the protease causing irreversible inhibition. C1 inactivator is one of only two known enzymic inhibitors of C1s, the other, nexin I, is far less effective. The mean serum concentration of C1 Inhibitor is 0.2g/L. C1 iInhibitorr consists of a single heavily glocosylated polypeptide chain with a molecule weight of 104kD.

Suitable for use in a variety of gel techniques including radial immunodiffusion (RID), double diffusion, immunoelectrophoresis (IEP) and rocket IEP. The use of 3% PEG 6000 with 1.2% agarose in a suitable buffer (such as TBE or Tris-barbital pH >8.2) is recommended. Other applications not tested.

Recommended Dilution:
RID: 2ul
Double Diffusion:10ul
IEP: 100ul
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and add glycerol (40-50%). Freeze at -20C. Aliquots are stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGHighly Purified
1ml4C (-20C Glycerol)Blue IceHumanSheep
~25mg/ml (OD280nm, E1% = 14.5)
Human C1 Inhibitor puri ed from plasma, shown to be homogeneous by SDS-PAGE.
Purified by ion-exchange chromatography (DEAE).
Supplied as a liquid in glycine buffered saline, pH 7.4, 0.09% sodium azide, 0.1% E-Amino-n-Caproic Acid, 0.01% Benzamidine, 1mM EDTA.
Single arc when tested by IEP and 2D-IEP against human plasma. Identity is con rmed by double diffusion (Ouchterlony) against human plasma and a known anti-human C1 Inhibitor.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.