Technical Data
C0013-32B
CBLB, ID (E3 Ubiquitin-protein Ligase CBL-B, Signal Transduction Protein CBL-B, SH3-binding Protein CBL-B, Casitas B-lineage Lymphoma Proto-oncogene b, RING Finger Protein 56, Nbla00127, RNF56)
|
Technical Data |
|
C0013-32B |
CBLB, ID (E3 Ubiquitin-protein Ligase CBL-B, Signal Transduction Protein CBL-B, SH3-binding Protein CBL-B, Casitas B-lineage Lymphoma Proto-oncogene b, RING Finger Protein 56, Nbla00127, RNF56) |
|
|
Description: E3 ubiquitin-protein ligase which accepts ubiquitin from specific E2 ubiquitin-conjugating enzymes, and transfers it to substrates, generally promoting their degradation by the proteasome. Negatively regulates TCR (T-cell receptor), BCR (B-cell receptor) and FCER1 (high affinity immunoglobulin epsilon receptor) signal transduction pathways. In naive T-cells, inhibits VAV1 activation upon TCR engagement and imposes a requirement for CD28 costimulation for proliferation and IL-2 production. It also acts by promoting PIK3R1/p85 ubiquitination, which impairs its recruitment to the TCR and subsequent activation. In activated T-cells, inhibits PLCG1 activation and calcium mobilization upon restimulation and promotes anergy. In B-cells, acts by ubiquitinating SYK and promoting its proteasomal degradation. It may also be involved in EGFR ubiquitination and internalization. Applications: Suitable for use in ELISA and Western Blot. Other applications not tested. Recommended Dilution: ELISA: 1:1,000 Western Blot: 1:100-1:500 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
|
|||||||||||
|
||||||||||||
|
||||||||||||
|
||||||||||||