Technical Data
c-erbB2, p185 (HER2, neu Receptor) (Glycerol, BSA & Azide Free)
c-erbB-2 is a membrane-associated oncoprotein having considerable sequence homology to EGFR. C-erbB-2 is the second member of the EGF receptor tyrosine kinase family of growth factor receptors. Overexpression of c-erbB-2/HER-2 is reportedly a marker of poor prognosis in node-positive and in defined subsets of node-negative breast cancer. Elevated levels of c-erbB-2/HER-2 has also been implicated in response to chemotherapy.

Suitable for ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation: 10ug/mg protein lysate (Use Protein A).
Western Blot: 5-10ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 2.5-5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
SKBR-3 cells, T47D cells or breast carcinomas.

Control Peptide:
C0015-30: c-erbB2, aa1243-1255, Control Peptide (HER2, neu Receptor)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
500ug-20CBlue IceHumanRabbit
Synthetic peptide aa1243-1255 (AENPEYLGLDVPV) derived from C-terminus of human c-erbB-2/HER-2 protein (KLH coupled). Sequence is identical in rat neu protein. Cellular Localization: Cell membrane. Epitope: C-terminal. MW of Antigen: 185kD.
Purified by Protein A affinity chromatography.
Supplied as a liquid in PBS, pH 7.4. No stablilizing proteins and no preservatives added. Available with BSA and sodium azide. See C0015. Also available without BSA and sodium azide. See C0015X.
Recognizes wild and mutant (oncogenic) forms of human c-erbB-2 protein (185kD). Does not crossreact with c-erbB-11 (EGF-R), c-erbB-3, or c-erbB-4. Species Crossreactivity: monkey, rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Yezhelyev, M.V. et al, (2007) Adv. Mater. 19:31463151. 1. Gullick, W.J., et al., International Journal of Cancer 40(2): 246-254 (1987).