Technical Data
C0015X1
c-erbB2, p185 (HER2, neu Receptor) (Glycerol, BSA & Azide Free)
Description:
c-erbB-2 is a membrane-associated oncoprotein having considerable sequence homology to EGFR. C-erbB-2 is the second member of the EGF receptor tyrosine kinase family of growth factor receptors. Overexpression of c-erbB-2/HER-2 is reportedly a marker of poor prognosis in node-positive and in defined subsets of node-negative breast cancer. Elevated levels of c-erbB-2/HER-2 has also been implicated in response to chemotherapy.

Applications:
Suitable for ELISA, Immunoprecipitation, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation: 10ug/mg protein lysate (Use Protein A).
Western Blot: 5-10ug/ml for 2 hours at RT.
Immunohistochemistry (Formalin/paraffin): 2.5-5ug/ml for 30 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0, for 10-20 minutes followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
SKBR-3 cells, T47D cells or breast carcinomas.

Control Peptide:
C0015-30: c-erbB2, aa1243-1255, Control Peptide (HER2, neu Receptor)

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
500ug-20CBlue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide aa1243-1255 (AENPEYLGLDVPV) derived from C-terminus of human c-erbB-2/HER-2 protein (KLH coupled). Sequence is identical in rat neu protein. Cellular Localization: Cell membrane. Epitope: C-terminal. MW of Antigen: 185kD.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4. No stablilizing proteins and no preservatives added. Available with BSA and sodium azide. See C0015. Also available without BSA and sodium azide. See C0015X.
Specificity:
Recognizes wild and mutant (oncogenic) forms of human c-erbB-2 protein (185kD). Does not crossreact with c-erbB-11 (EGF-R), c-erbB-3, or c-erbB-4. Species Crossreactivity: monkey, rat and mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
US Biological application reference: Yezhelyev, M.V. et al, (2007) Adv. Mater. 19:31463151. 1. Gullick, W.J., et al., International Journal of Cancer 40(2): 246-254 (1987).