		Technical Data
C0026-11N1	c-erbB2 (HER-2, neu Receptor)

Description:
c-erbB-2 is the cellular homolog of the neu oncogene. c-erbB-2 is comprised of an extracellular ligand binding domain, a single transmembrane domain, a tyrosine kinase domain, and a C terminal region which mediates signaling. c-erbB-2 forms heterodimers with other members of the EGF receptor family, such as c-erbB-1. In response to ligand binding, c-erbB-2 is autophosphorylated on five tyrosine residues at its C terminus, producing docking sites for SH2-domain containing proteins such as phospholipase C , PI3-K, Src, CHK, and others. Over-expression of c-erbB-2 is observed in 15-40% of primary human breast tumors, and correlates with poor prognosis in node positive breast cancer. c-erbB-2 over-expression is also observed in ovarian, gastric, salivary, and non-small cell lung carcinomas.

Applications:
Suitable for use in Immunoprecipitation, Western Blot, ELISA, Flow Cytometry and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunoprecipitation (native and denatured): 2ug/mg cell lysate
Western Blot: 1-2ug/ml with 2 hour incubation
Immunohistochemistry (Formalin fixed paraffin embedded): 2-4ug/ml with 30 minute incubation. Requires boiling the sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, cool at RT for 10 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:
SKBR-3 cells (breast cancer cell line), T47D cells.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	5J297	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Cytoplasmic domain of human c-erbB-2 protein. Cellular Localization: Cell membrane.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in PBS, pH 7.4. Preservative free.

Specificity:

Recognizes human c-erbB-2, a receptor protein tyrosine kinase with Mr=185kD, which is also known as p185HER-2. Species Crossreactivity: mouse and rat.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Tandon, A.K., G.M. Clark, G.C. Chamness, A. Ullrich, and W.L. McGuire (1989) HER-2/neu oncogene protein and prognosis in breast cancer. J. Clin. Oncol. 7:1120-1128. 2. Slamon, D.J., W. Godolphin, L.A. Jones, J.A. Holt, S.G. Wong, D.E. Keith, W.J. Levin, S.G. Stuart, J. Udove, A. Ullrich, et al. (1989) Studies of the HER-2/neu proto-oncogene in human breast and ovarian cancer. Science 244:707-712.