Technical Data
C0045-75B
C4.4A (LYPD3, Y6/PLAUR domain containing 3 GPI-anchored metastasis-associated protein C4.4A homolog, Ly6/PLAUR domain-containing protein 3 precursor, Matrigel-induced gene C4 protein, MIG-C4, UNQ491/PRO1007)
Description:
C4.4A (Ly6/PLAUR-domain containing protein 3; also MIG-C4 and GPI-anchored metastasis-associated protein C4.4A) is an 85-95kD, variably glycosylated member of the Ly6 family of GPI-anchored proteins. It is expressed on transitional epithelium, keratinocytes and monocytes, and binds laminin 1 and 5, plus Galectin-3. C4.4A is believed to support cell migration.

Applications:
Suitable for Western Blot, Immunohistochemistry and ELISA. Other applicatons not tested.

Recommended Dilution:
Western Blot: 1ug/ml. Tissue lysates were resolved by SDS-PAGE, transferred to an Immobilon-P membrane and immunoblotted.
Immunohistochemistry: 2-15ug/ml will detect C4.4A in cells and tissues.
Direct ELISA: 0.5-1ug/ml. The detection limit is ~0.3ng/well.
Optimal dilutions should be determined by each laboratory for each application.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile PBS. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceMouseGoat
Concentration:
~0.2mg/ml (after reconstitution)
Immunogen:
Recombinant corresponding to mouse C4.4A expressed in NS0 cells (Q91YK8).
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a lyophilized powder from PBS, 5% trehalose. Reconstitute with 500ul sterile PBS.
Specificity:
Recognizes mouse C4.4A. In direct ELISA, this antibody shows approximately 40% cross-reactivity with rhC4.4A/LYPD3.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.