Technical Data
C0050-16
CA125 Cancer Antigen, B Domain (Ovarian Cancer)
Description:
MUC16/CA125 antigen is a serum marker that is used routinely in gynecologic practice to monitor patients with ovarian cancer. It is a mullerian duct differentiation antigen that is overexpressed in epithelial ovarian cancer cells and secreted into the blood, although its expression is not entirely confined to ovarian cancer. Most biochemical studies have concluded that MUC16/CA125 is a high molecular mass glycoprotein.

According to the ISOBM-TD-1 workshop, CA125 contains three antigenic domains: A-domain (Oc125), B-domain (M11), C-Domain (Ov197). The CA-125 antigen is present on a mucin-like glycoprotein of >200kD and is detectable on the apical surface of normal endometrium and endocervix.

Applications:
Suitable for use in ELISA, Western Blot and Immunohistochemistry. Other applications have not been tested.

Recommended Dilutions:
Immunohistochemistry: Paraffin-embedded tissues
Optimal dilutions to be determined by the researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Source:
Ascites

Matched Pair:
Detection: C0050-16
Capture: C0050-13

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG11.B.830Highly Purified
SizeStorageShippingSourceHost
1mg-20CBlue IceHumanMouse
Concentration:
~7mg/ml
Immunogen:
CA125 antigen purified from human ovarian carcinoma (MW >1000kD)
Purity:
Purified by Ion Exchange Chromatography.
Form
Supplied as a liquid in 10mM Tris, 0.15M sodium chloride, pH 7.5, 0.1% sodium azide.
Specificity:
Recognizes B-domain in the protein core of the human CA125 ovarian cancer antigen.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.