Technical Data
C0104-56
CABP (CABP1, CALBRAIN, Calcium Binding Protein 1, Calcium Binding Protein 5, HCALB_BR)
Description:
The protein belongs to a subfamily of calcium binding proteins, which share similarity to calmodulin. Calcium binding proteins are an important component of calcium mediated cellular signal transduction. This protein is only detected in retina and brain. Study of the mouse homolog demonstrated that groups of cells expressing this protein are located in the center or inner border of the inner unclear layer of retina. Two alternatively spliced variants encoding different isoforms have been described.

Applications:
Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5-2.0ug/ml; ~20kD band seen in Human Brain lysate (predicted MW 19kD).
Optimal dilutions to be determined by the researcher.

Positive Control:  
Human Brain lysate
 
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgG Affinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceHumanGoat
Concentration:
~0.5mg/ml
Immunogen:
Synthetic peptide [ GNCVKYPLRNLS], from the N Terminus of the protein sequence according to NP_004267; NP_112482.
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in TBS, pH 7.3, 0.5% BSA, 0.02% sodium azide.
Specificity:
Expected to recognize both isoforms of CABP.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Yamaguchi K et al. Calbrain, a novel two EF-hand calcium-binding protein that suppresses Ca2+/calmodulin-dependent protein kinase II activity in the brain. J Biol Chem 274 :3610-6 (1999). PubMed: 9920909.