Technical Data
C0104-76B
CACNB2 (Calcium Channel Voltage-dependent beta 2 Subunit, CAB2, CACNLB2, CAVB2, FLJ23743, Lambert-Eaton Myasthenic Syndrome Antigen B, Myasthenic (Lambert-Eaton) Syndrome Antigen B, MYSB, Voltage-dependent L-type Calcium Channel Subunit beta 2)
Description:
Ion channels are integral membrane proteins that help establish and control the small voltage gradient across the plasma membrane of living cells by allowing the flow of ions down their electrochemical gradient (1). They are present in the membranes that surround all biological cells because their main function is to regulate the flow of ions across this membrane. Whereas some ion channels permit the passage of ions based on charge, others conduct based on a ionic species, such as sodium or potassium. Furthermore, in some ion channels, the passage is governed by a gate which is controlled by chemical or electrical signals, temperature, or mechanical forces. There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand-gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium
channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels (2).
Cav Beat subunits are involved in the transport of the pore forming alpha1 subunit to the plasma membrane (3). They also shield an ER Retention signal on the alpha1 subunit, thereby guiding the pore-forming subunit to the target membrane (4, 5). They also determine the biophysical
properties of the calcium channel (5).

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot:1-10ug/ml
Immunohistochemistry:0.1-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110F683Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide aa 189-205 of rat Cavb2 (RSPKPSANSVTSPHSKE; accession number NP_446303).
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, pH7.4, 50% glycerol and 0.09% sodium azide.
Specificity:
Recognizes rat Cavb2. Species Crossreactivity: human. No cross reactivity against Cavb1, Cavb3, Cavb4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Dolphin A.C. (2003) J Bioenerg. Biomembr. 35:599-620. 4. Bichet D., et al. (2000) Neuron. 25:177-190. 5. Xie M., et al. (2007) J Cell Biol. 178(3):489-502.