Technical Data
C0104-76B
CACNB2 (Calcium Channel Voltage-dependent beta 2 Subunit, CAB2, CACNLB2, CAVB2, FLJ23743, Lambert-Eaton Myasthenic Syndrome Antigen B, Myasthenic (Lambert-Eaton) Syndrome Antigen B, MYSB, Voltage-dependent L-type Calcium Channel Subunit beta 2)
Description:
There are a few main classifications of gated ion channels. There are voltage- gated ion channels, ligand-gated, other gating systems and finally those that are classified differently, having more exotic characteristics. The first are voltage- gated ion channels which open and close in response to membrane potential. These are then separated into sodium, calcium, potassium, proton, transient receptor, and cyclic nucleotide-gated channels; each of which is responsible for a unique role. Ligand-gated ion channels are also known as ionotropic receptors, and they open in response to specific ligand molecules binding to the extracellular domain of the receptor protein. The other gated classifications include activation and inactivation by second messengers, inward-rectifier potassium channels, calcium-activated potassium channels, two-pore-domain potassium channels, light-gated channels, mechano-sensitive ion channels and cyclic nucleotide-gated channels. Finally, the other classifications are based on less normal characteristics such as two-pore channels, and transient receptor potential channels.
Cav Beat subunits are involved in the transport of the pore forming alpha1 subunit to the plasma membrane.

Applications:
Suitable for use in Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1ug/ml
Immunohistochemistry: 0.1-1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG110F683Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to aa189-205, RSPKPSANSVTSPHSKE from rat CACNB2 (NP_446303).
Purity:
Purified by Protein G affinity chromatography
Form
Supplied as a liquid in PBS, pH7.4, 0.09% sodium azide, 50% glycerol
Specificity:
Recognizes rat CACNB2 at ~78kD. Species Crossreactivity: human and mouse. No cross reactivity against CavB1, CavB3, CavB4.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Hille B. (2001) Ion Channels of Excitable Membranes, 3rd Ed., Sinauer Associated Inc.: Sunderland, MA USA. 2. www.iochannels.org 3. Dolphin A.C. (2003) J Bioenerg. Biomembr. 35:599-620. 4. Bichet D., et al. (2000) Neuron. 25:177-190. 5. Xie M., et al. (2007) J Cell Biol. 178(3):489-502.