Technical Data
CACNB4 (Calcium channel, voltage-dependent, beta 4 subunit; CAB4; CACNLB4; EA5; EJM; dihydropyridine-sensitive L-type; calcium channel beta-4 subunit; voltage dependent calcium channel beta 4 subunit)
This gene encodes a member of the beta subunit family, a protein in the voltage-dependent calcium channel complex. Calcium channels mediate the influx of calcium ions into the cell upon membrane polarization and consist of a complex of alpha-1, alpha-2/delta, beta, and gamma subunits in a 1:1:1:1 ratio. Various versions of each of these subunits exist, either expressed from similar genes or the result of alternative splicing. The protein described in this record plays an important role in calcium channel function by modulating G protein inhibition, increasing peak calcium current, controlling the alpha-1 subunit membrane targeting and shifting the voltage dependence of activation and inactivation. Certain mutations in this gene have been associated with idiopathic generalized epilepsy (IGE) and juvenile myoclonic epilepsy (JME). Alternate transcriptional splice variants of this gene, encoding different isoforms, have been characterized.

Positive Control: Human Bone Marrow lysate

Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:4000.
Western Blot: 0.2-0.6ug/ml. ~ 55-60kD band observed in Human Bone Marrow lysates (calculated MW of 54.7kD according to NP_001005747.1 and 58.2kD according to NP_000717.2).
Optimal dilutions to be determined by the researcher.

Storage and Stability:

May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanGoat
Synthetic peptide, C-DYPDSYQDTYKPH, representing the internal region (near the C Terminus) of the human protein according to NP_001005747.1; NP_000717.2; NP_001005746.1. Homology: Human, Mouse, Rat, Dog Epitope: DYPDSYQDTYKPH
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-saline, pH7.2, 0.5% BSA.
Species Crossreactivity: Human. Other species not tested.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Escayg A, Jones JM, Kearney JA, Hitchcock PF, Meisler MH. Calcium channel beta 4 (CACNB4): human ortholog of the mouse epilepsy gene lethargic. Genomics. 1998 May 15;50(1):14-22. PMID: 9628818