		Technical Data
C0107-06	Cadherin 11 (OB-Cadherin, Osteoblast, CDH11)

Description:
Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers.Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin orcadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is basedon the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:25-1:250
Immunohistochemistry (Frozen): 1:25-1:50. Use PBS, 0.1mM calcium chloride, 0.1mM magnesium chloride. Recommended Detecting Agent: Avidin-biotinylated horseradish peroxidase complex (ABC).
Optimum dilutions to be determined by researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	4A11	Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Mouse

Concentration:

~1mg/ml

Immunogen:

Affinity purified extracellular domain of human cadherin-11-GSTfusion protein.

Purity:

Purified

Form

Supplied as a liquid in PBS, 0.09% sodium azide.

Specificity:

Recognizes the extracellular domain of human Cadherin 11 (OB-cadherin). Species Crossreactivity: rat

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Bussemakers, M.J., et al., Complex cadherin expression in human prostate cancer cells, Int J Cancer 85: 446-50 (2000). 2. Tomita, K., et al., caherin switching in human prostate cancer progression, Cancer Res 60: 3650-4 (2000).