Technical Data
C0107-06
Cadherin 11 (OB-Cadherin, Osteoblast, CDH11)
Description:
Cadherins constitute a family of transmembrane glycoproteins involved in Ca2+-dependent cell-cell interactions. The members of this family are differentially expressed in various tissues. The cadherins generally contain five extracellular repeats, a transmembrane domain and a cytoplasmic tail that binds to the catenin family of cytoskeletal anchoring proteins which also function as signal transducers.Cadherins are divided into type I and type II subgroups. Type I cadherins include epithelial cadherin (E-cadherin, cadherin-1 or uvomorulin), neural cadherin (N-cadherin or cadherin-2), placental cadherin (P-cadherin orcadherin-3) and retinal cadherin (R-cadherin or cadherin-4). Kidney cadherin (K-cadherin or cadherin-6) and osteoblast cadherin (OB-cadherin or cadherin-11) are type II cadherins. The progression of carcinomas is associated with the loss of epithelial morphology and a concomitant acquisition of a more mesenchymal phenotype, which is thought to contribute to the invasive and/or metastatic behavior. A putative role for cadherin-11 in these late stages of tumor progression is basedon the fact that migration of mesenchymal cells is facilitated when cadherin-11 is highly expressed.

Applications:
Suitable for use in Western Blot, Immunocytochemistry and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Western Blot: 1:25-1:250
Immunohistochemistry (Frozen): 1:25-1:50. Use PBS, 0.1mM calcium chloride, 0.1mM magnesium chloride. Recommended Detecting Agent: Avidin-biotinylated horseradish peroxidase complex (ABC).
Optimum dilutions to be determined by researcher.

Hybridoma:
Sp2/0 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

TypeIsotypeCloneGrade
MabIgG14A11Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~1mg/ml
Immunogen:
Affinity purified extracellular domain of human cadherin-11-GST fusion protein.
Purity:
Purified
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes the extracellular domain of human Cadherin 11 (OB-cadherin). Species Crossreactivity: rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bussemakers, M.J., et al., Complex cadherin expression in human prostate cancer cells, Int J Cancer 85: 446-50 (2000). 2. Tomita, K., et al., caherin switching in human prostate cancer progression, Cancer Res 60: 3650-4 (2000).