Technical Data
C0108-26F
CDHE (Cadherin-1, CAM 120/80, Epithelial Cadherin, E-cadherin, Uvomorulin, CD324, CDH1, UVO)
Description:
Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with B-catenin, Y-catenin (also called plakoglobin), and p120 catenin. B-catenin and Y-catenin associate with A-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Unlike B- and Y-catenin, p120 regulates cadherin adhesive activity and trafficking rather than having a structural role in the junctional complex (1-4). E-cadherin is considered an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to over-expression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).

Applications:
Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG132A8Ascites
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanMouse
Concentration:
Not determined
Immunogen:
Human cancer cell lines.
Purity:
Ascites
Form
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
Specificity:
Recognizes endogenous levels of total human E-cadherin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Wheelock, M.J. and Johnson, K.R. (2003) Annu. Rev. Cell. Dev. Biol. 19, 207-235. 2.Christofori, G. (2003) EMBO J. 22, 2318-2323. 3.Hazan, R.B. et al. (2004) Ann. NY Acad. Sci. 1014, 155-163. 4.Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol. 14, 427-434. 5.Rabascio, C. et al. (2004) Cancer Res. 64, 4373-4377. 6.Yamaoka-Tojo, M. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26, 1991-1997. 7.Patel, I.S. et al. (2003) Int. J. Cancer 106, 172-177. 8.Sanders, D.S. et al. (2000) J. Pathol. 190, 526-530.