Technical Data
CDHE (Cadherin-1, CAM 120/80, Epithelial Cadherin, E-cadherin, Uvomorulin, CD324, CDH1, UVO)
Cadherins are a superfamily of transmembrane glycoproteins that contain cadherin repeats of approximately 100 residues in their extracellular domain. Cadherins mediate calcium-dependent cell-cell adhesion and play critical roles in normal tissue development (1). The classic cadherin subfamily includes N-, P-, R-, B- and E-cadherins as well as about ten other members that are found in adherens junctions, a cellular structure near the apical surface of polarized epithelial cells. The cytoplasmic domain of classical cadherins interacts with B-catenin, Y-catenin (also called plakoglobin), and p120 catenin. B-catenin and Y-catenin associate with A-catenin, which links the cadherin-catenin complex to the actin cytoskeleton (1,2). Unlike B- and Y-catenin, p120 regulates cadherin adhesive activity and trafficking rather than having a structural role in the junctional complex (1-4). E-cadherin is considered an active suppressor of invasion and growth of many epithelial cancers (1-3). Recent studies indicate that cancer cells have up-regulated N-cadherin in addition to loss of E-cadherin. This change in cadherin expression is called the "cadherin switch." N-cadherin cooperates with the FGF receptor, leading to over-expression of MMP-9 and cellular invasion (3). In endothelial cells, VE-cadherin signaling, expression, and localization correlate with vascular permeability and tumor angiogenesis (5,6). Expression of P-cadherin, which is normally present in epithelial cells, is also altered in ovarian and other human cancers (7,8).

Suitable for use in Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Western Blot: 1:1000
Immunoprecipitation: 1:200
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
100ul-20CBlue IceHumanMouse
Not determined
Human cancer cell lines.
Supplied as a liquid in 10mM sodium HEPES, pH 7.5, 150mM sodium chloride, 100ug/ml BSA, <0.02% sodium azide, 50% glycerol.
Recognizes endogenous levels of total human E-cadherin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Wheelock, M.J. and Johnson, K.R. (2003) Annu. Rev. Cell. Dev. Biol. 19, 207-235. 2.Christofori, G. (2003) EMBO J. 22, 2318-2323. 3.Hazan, R.B. et al. (2004) Ann. NY Acad. Sci. 1014, 155-163. 4.Bryant, D.M. and Stow, J.L. (2004) Trends Cell Biol. 14, 427-434. 5.Rabascio, C. et al. (2004) Cancer Res. 64, 4373-4377. 6.Yamaoka-Tojo, M. et al. (2006) Arterioscler. Thromb. Vasc. Biol. 26, 1991-1997. 7.Patel, I.S. et al. (2003) Int. J. Cancer 106, 172-177. 8.Sanders, D.S. et al. (2000) J. Pathol. 190, 526-530.