Technical Data
C0108-31B
Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)
Description:
One of the epithelial cell adhesion molecules, E-Cadherin, plays an important role in the formation of cell-cell contacts in epithelia irrespective their origin form ecto-, meso- or endodermal tissue. This early adhesion event between epithelial cells is a prerequisite for the assembly of all elements of the junctional complex. Furthermore, E-Cadherin plays a crucial role in the maintenance of the epithelial junctional complex and is as such an important molecule in maintaining epithelial integrity. Over 90% of the malignant tumors are carcimomas. One of the prerequisites for the release of carcinoma cells from the primary site might be a defect in intercellular adhesion mediated by the absence of E-Cadherin expression. Therefore, the expression of E-Cadherin might be an important parameter for the determination of the invasive potential of epithelial neoplasms, and for the transition of a benign to a malignant neoplasm.

Applications:
Suitable for use in ELISA, Flow Cytometry, Western Blot, Immunohistochemistry and Inhibition. Other applications not tested.

Recommended Dilution:
ELISA: 2ug/ml
Flow Cytometry: 1ug/ml
Western Blot: 2ug/ml; reducing and non-reducing conditions
Immunohistochemistry (Frozen, paraffin): 2ug/ml; Incubate overnight at 4C.
Inhibition: 1ug/ml inhibits E-cadherin-dependent cell-cell contact

Hybridoma:
P3X63-Ag8-U1 myeloma cells with spleen cells from Balb/c mice.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2a6D185Highly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~2mg/ml
Immunogen:
Soluble E-cadherin from human placenta.
Purity:
Purified by ammonium sulfate precipitation and ion exchange chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.4,1% BSA. No preservative added. Reconstitute in 50ul sterile ddH2O. Dilute further in 20mM TBS, pH 7.4, 10mM calcium chloride, 1% BSA.
Specificity:
Recognizes human E-cadherin. Does not cross-react with rat E-cadherin.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Soluble E-cadherin fragments increased in circulation of cancer patients: M. Katayama, et al.; Br. J. Cancer 69, 580 (1994)
2. Induction of polarized cell-cell association and retardation of growth by activation of the E-cadherin-catenin adhesion system in a dispersed carcinoma line: M. Watabe, et al.; J. Cell. Biol. 127, 247 (1994)
3. Soluble fragment of E-cadherin cell adhesion molecule increase in urinary excretion of cancer patients, potentially indicating its shedding from epithelial tumour cells: M. Katayama, et al.; Int. J. Oncol. 5, 1049 (1994)