		Technical Data
C0108-33	Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1)

Description:
Cdh1 protein is a limiting, substratespecific activator of anaphase-promoting complex (APC)-dependent proteolysis and it is required for the degradation of the APC substrates, such as Clb2 and Ase1. Cdh1 plays important role in mitotic regulation.

Applications:
Suitable for use in Immunoprecipitation and Western Blot. Other applications have not been tested.

Recommended Dilution:
Immunoprecipitation (Denatured verified): 2ug/mg protein lysate. Use Protein G
Western Blot: 1-2ug/ml for 2hrs at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
Jurkat or LS174T cells.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1,k	3F40	Affinity Purified

Size		Storage	Shipping	Source	Host
500ul		-20°C	Blue Ice	Human	Mouse

Concentration:

~0.2mg/ml

Immunogen:

Recombinant human cdh1 protein. MW of Antigen: ~55kD

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in 10mM PBS, pH 7.4, 0.2% BSA, 0.09% sodium azide. Also available without BSA and azide. See C0108-33X.

Specificity:

Recognizes human Cadherin E (Cadherin 1, E-Cadherin, L-CAM, Epithelial Cell Adhesion Molecule, Uvomorulin, CDH1).

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1.Kramer ER, Scheuringer N, Podtelejnikov AV, Mann M, Peters JM. Mol Biol Cell 2000 May; 11(5): 1555–69. 2. Pfleger CM, Kirschner MW. Genes Dev 2000 Mar 15; 14(6):655–65. 3. Visintin R, Prinz S, Amon A. Science 1997 Oct 17; 278 (5337): 460–3.