Technical Data
C0113-02B
Calbindin
Description:
Calbindin is a calcium-binding protein that functions as a buffer of cytosolic calcium and is found in brain, kidney, gut and pancreatic islets. Altered expression of calbindin has been reported in certain conditions such as Huntington’s disease. This modified pattern may indicate changes in Ca2+-mediated functions. This antibody will help detect calbindin and calbindin expressing tissues.

Applications:
Suitable for use in Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:100-1:200 for 1 hour at RT
Optimal dilutions to be determined by the researcher.

Positive Control:
Tonsil, cytoplasmic staining pattern

Storage and Stability:
Lyophilized powder may be stored at -20°C. Stable for 12 months at -20°C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG1KR6Supernatant
SizeStorageShippingSourceHost
1ml-20°CBlue IceMouse
Concentration:
Not determined
Immunogen:
Recombinant protein representing the majority of the calbindin.
Purity:
Supernatant
Form
Supplied as a lyophilized powder in 15mM sodium azide. Reconstitute with 1ml of dH2O.
Specificity:
Recognizes Calbindin. Species Crossreactivity: Human.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Nag T C and Wadhwa S. Calbindin immunoreactivity in the developing and adult human cerebellum. Journal of Chemical Neuroanatomy. 17(1):1-12 (1999). 2.Tsunoda T, Yamakawa M and Takahashi T. Differential expression of Ca2+-binding proteins on follicular dendritic cells in non-neoplastic and neo- plastic lymphoid follicles. American Journal of Pathology 155(3):805-814 (1999). 3.Chilosi M, Doglioni C, Pelosio P, et al.. Detection of calbindin-D immunoreactivity in human follicular dendritic cells. Journal of Pathology 166:121-127 (1992). 4.Ito H, Goto S, Sakamoto S, et al.. Calbindin-D28k in the basal ganglia of patients with parkinsonism. Annals of Neurology 32(4):543-550 (1992).