Technical Data
C0114-12
CALNA3, NT (Calmodulin-dependent Calcineurin A Subunit gamma Isoform, Serine/Threonine-protein Phosphatase 2B Catalytic Subunit gamma Isoform, CAM-PRP Catalytic Subunit, Calcineurin, Testis-specific Catalytic Subunit, PPP3CC, CNA3)
Description:
Calmodulin-dependent protein phosphatase, calcineurin, is involved in a wide range of biologic activities, acting as a Ca(2+)-dependent modifier of phosphorylation status. In testis, the motility of the sperm is thought to be controlled by cAMP-dependent phosphorylation and a unique form of calcineurin appears to be associated with the flagellum. The calcineurin holoenzyme is composed of catalytic and regulatory subunits of 60 and 18kD, respectively. At least 3 genes, calcineurin A-alpha, calcineurin A-beta, and calcineurin A-gamma (CALNA3), have been cloned for the catalytic subunit. These genes have been identified in humans, mice, and rats, and are highly conserved between species (90 to 95% amino acid identity).

Applications:
Suitable for use in ELISA, Western Blot, and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
ELISA: 1:1,000
Western Blot: 1:100-1:500
Immunohistochemistry: 1:50-1:100
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGPurified
SizeStorageShippingSourceHost
200ul-20CBlue IceHumanRabbit
Concentration:
As reported
Immunogen:
Synthetic peptide selected from the N-terminal region of human PPP3CC (KLH).
Purity:
Purified by ammonium sulfate precipitation.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes human PPP3CC.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1.Eastwood, S.L., et al., Biol. Psychiatry 57(7):702-710 (2005). 2.Gerber, D.J., et al., Proc. Natl. Acad. Sci. USA 100(15):8993-8998 (2003). 3.Bennasser, Y., et al., Virology 303(1):174-180 (2002). 4.Esau, C., et al., J. Exp. Med. 194(10):1449-1459 (2001). 5.Muramatsu, T., et al., Biochem. Biophys. Res. Commun. 188(1):265-271 (1992).