Technical Data
C0115-01D
Calcitonin Gene Related Peptide (CGRP, Calcitonin Gene-related Peptide 1, CGRP1, Calcitonin Gene-related Peptide I, CGRP I, CGRP-I, Alpha Type CGRP, CAL6, Cal1, Calc, Calcitonin 1, CALC 1, CALC 2, RATCAL6, Calcitonin-related Polypeptide alpha, CALC A, CAL
Description:
CGRP is present in C-cells of the thyroid and in central and peripheral nerves. CGRP has several important physiologic roles: it is a potent vasodilator, and can affect the force and rate of heart beat; it can modulate acetylcholine receptor function at the neuromuscular junction; it has been demonstrated to block tolerance to morphine; and it can modulate antigen presentation in Langerhans cells in the skin. Despite these important physiologic functions, therapeutic strategies using CGRP have been impeded due to the lack of a cloned CGRP receptor with which ligands could be developed.

Applications:
Suitable for use in ELISA, Immunofluorescence and Immunohistochemistry. Not recommended for frozen or resin sections. Other applications not tested.

Recommended Dilutions:
ELISA: 1:500-1:2500
Immunohistochemistry: Paraffin sections
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-28K: IgG, H&L (HRP) Pab RbxGt
I1904-28Q: IgG, H&L, X-Adsorbed (HRP) Pab RbxGt

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ul-20CBlue IceRatGoat
Concentration:
~5mg/ml
Immunogen:
Synthetic peptide corresponding to aa23-37 of rat Tyr-CGRP conjugated to gamma globulin.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a liquid in PBS, 0.09% sodium azide.
Specificity:
Recognizes rat Calcitonin gene-related peptide, also known as CGRP. Reacts with both the whole molecule (aa1-37) and the C-terminal fragment (23-37). Species Crossreactivity: mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Collins, J.J. et al (2000) Distribution and origin of secretoneurin-immunoreactive nerves in the female rat uterus. Neuroscience 95: 255-264.2. Pritz. M.B. and Stritzel, M.E. (1988) Thalamic nuclei that project to reptilian telencephalon lack GABA and GAD immunoreactive neurons and puncta. Brain Res. 457: 154-159. 3. Pritz. M.B. and Stritzel, M.E. (1989) Reptilian dorsal column nucleus lacks GAD immunoreactive neurons. Brain Res. 503: 175-179.4. Fan, W. et al. (2010) Structural and cellular features in metaphyseal and diaphyseal periosteum of osteoporotic rats. J Mol Histol. 41: 51-60.5. Hamed, K. et al. (2011) Changes in cutaneous innervation in patients with chronic pain after burns. Burns. Jan 21. 6. Brock, J.A. et al. (2007) Postnatal androgen deprivation dissociates the development of smooth muscle innervation from functional neurotransmission in mouse vas deferens. J Physiol. 581: 665-78