Technical Data
Calmodulin (Phosphodiesterase 3'-5'-Cyclic Nucleotide Activator)
Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions.

Suitable for use in Western Blot, Immunoprecipation and RIA. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.1-1ug/ml detects calmodulin (~17kD) in RIPA lysates from human A431 carcinoma cells. Previous lots detected calmodulin in RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C1035-05. Proteins were visualized using a Gt xMo IgG (HRP) and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitates calmodulin from A431 RIPA cell lysate.
RIA: 10ug/ml detects calmodulin in the range of 200-300ng.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(ab)2 (HRP) Pab Gt xMo

Included Positive Antigen Control:
A0001-50: Non-stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
MabIgG10.T.172Affinity Purified
100ug-20CBlue IceBovineMouse
Synthetic peptide corresponding to the 21 carboxy-terminal amino acids (aa128-148) of bovine calmodulin, conjugated with tyroglobulin.
Purified by Protein G affinity chromatography.
Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.
Recognizes both Ca 2+-bound and Ca 2+-free bovine calmodulin. Does not recognize bovine S100- alpha, S100-beta, troponin C or parvalbumin. Species crossreactivity: rat, mouse, human, porcine and wheat germ. Wide crossreactivity expected.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
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3. Kapus, A., et al. (1999). J Biol Chem. 274: 8093-102. 4. Lee, A., et al. (1999). Nature. 399: 155-9. 5. Deisseroth, K., et al. (1998). Nature. 392: 198-202.