		Technical Data
C1035-05	Calmodulin (Phosphodiesterase 3'-5'-Cyclic Nucleotide Activator)

Description:
Calmodulin (CaM) is a ubiquitous, calcium-binding protein that can bind to and regulate a multitude of different protein targets, thereby affecting many different cellular functions.

Applications:
Suitable for use in Western Blot, Immunoprecipation and RIA. Other applications not tested.

Recommended Dilutions:
Western Blot: 0.1-1ug/ml detects calmodulin (~17kD) in RIPA lysates from human A431 carcinoma cells. Previous lots detected calmodulin in RIPA lysates from mouse 3T3 and L6 rat skeletal fibroblasts. Non-stimulated A431 cell lysate was resolved by electrophoresis, transferred to nitrocellulose and probed with C1035-05. Proteins were visualized using a Gt xMo IgG (HRP) and a chemiluminescence detection system.
Immunoprecipitation: 4ug immunoprecipitates calmodulin from A431 RIPA cell lysate.
RIA: 10ug/ml detects calmodulin in the range of 200-300ng.
Optimal dilutions to be determined by the researcher.

Recommended Secondary Antibodies:
I1904-06C: IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-06H IgG, H&L (HRP) (X-Adsorbed) Pab Gt xMo
I1904-14C: IgG, Fc (HRP) Pab Gt xMo
I1904-19C: IgG, F(ab’)2 (HRP) Pab Gt xMo

Included Positive Antigen Control:
A0001-50: Non-stimulated A431 cell lysate. Add 2.5ul of 2-mercaptoethanol/100ul of lysate and boil for 5 minutes to reduce the preparation. Load 20ug of reduced lysate per lane for minigels.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Mab	IgG1	0.T.172	Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Bovine	Mouse

Concentration:

~1mg/ml

Immunogen:

Synthetic peptide corresponding to the 21 carboxy-terminal amino acids (aa128-148) of bovine calmodulin, conjugated with tyroglobulin.

Purity:

Purified by Protein G affinity chromatography.

Form

Supplied as a liquid in 0.1M Tris-glycine, pH 7.4, 0.15M sodium chloride, 0.05% sodium azide.

Specificity:

Recognizes both Ca 2+-bound and Ca 2+-free bovine calmodulin. Does not recognize bovine S100- alpha, S100-beta, troponin C or parvalbumin. Species crossreactivity: rat, mouse, human, porcine and wheat germ. Wide crossreactivity expected.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Xu, Jing, et al. (2007). Hippocampus. 17: 525- 37. 2. Liang, L., et al. (2000). J Biol Chem. 275: 30075-81.
3. Kapus, A., et al. (1999). J Biol Chem. 274: 8093-102. 4. Lee, A., et al. (1999). Nature. 399: 155-9. 5. Deisseroth, K., et al. (1998). Nature. 392: 198-202.