		Technical Data
C1035-25B3	CAMK2A (Calcium/Calmodulin-Dependent Protein Kinase (CaM kinase) II Alpha, CAMKA, KIAA0968, CaM Kinase II alpha Subunit, CaM-kinase II alpha Chain; CaMK-II alpha Subunit, Calcium/Calmodulin-Dependent Protein Kinase II Alpha-B Subunit, Calcium/Calmodulin-D

Description:
CaMKII is an important member of calcium/calmodulin-activated protein kinase family, functioning in neural synaptic stimulation and T-cell receptor signaling. CaMKII has catalytic and regulatory domains. The binding of Ca++/calmodulin to its regulatory domain releases its autoinhibitory effect and activates the kinase. The activated CaMKII further autophosphorylates at Thr286 to render the kinase constitutively active. The threonine phosphorylation state of CaMKII can be regulated through PP1/PKA. PP1 (protein phosphatase 1) dephosphorylates phospho-CaMKII at Thr286. PKA (protein kinase A) prevents this dephosphorylation through its inhibitory effect on PP1.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilution:
ELISA: 1:64,000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Type	Isotype	Clone	Grade
Pab	IgG		Affinity Purified

Size		Storage	Shipping	Source	Host
100ug		-20°C	Blue Ice	Human	Goat

Concentration:

~0.5mg/ml

Immunogen:

Synthetic peptide corresponding to C-PRTAQSEETRVWHR, from the internal region of human Camk2a (NP_057065.2; NP_741960.1).

Purity:

Purified by affinity chromatography.

Form

Supplied as a liquid in Tris saline, 0.02% sodium azide, pH7.3, 0.5% BSA.

Specificity:

Recognizes human Camk2a. Species crossreactivity: mouse. Sequence homology: rat, canine.

Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.

1. Lee CW, Lin CC, Luo SF, Lee HC, Lee IT, Aird WC, Hwang TL, Yang CM. Tumor necrosis factor-alpha enhances neutrophil adhesiveness: induction of vascular cell adhesion molecule-1 via activation of Akt and CaM kinase II and modifications of histone acetyltransferase and histone deacetylase 4 in human tracheal smooth muscle cells. Molecular pharmacology 2008 May 73 (5): 1454-64.