Technical Data
Calnexin - ER Marker
Cellular Localization: Endoplasmic reticulum

Positive Control: Heat Shocked HeLa Cell Lysate.

Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry: 1:100
Immunocytochemistry: 1:200
Immunoprecipitation: 1:100
Western Blot 1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
100ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide: DTSAPTSPKVTYKAPVPSG conjugated to KLH, corresponding to amino acids 50-68 of Human Calnexin.
Whole Serum. No preservative
Species Crossreactivity: Crossreacts with Rat, Xenopus laevis, bovine, Chicken, Drosophilia melanogaster, Guinea pig, Hamster, Pig, Quail, Rabbit and Sheep. Expected to crossreact with Human (100% identity with immunogen), Mouse (100% identity with immunogen) and canine (100% identity with immunogen) due to sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Hammond C & Helenius A Quality control in the secretory pathway: retention of a misfolded viral membrane glycoprotein involves cycling between the ER, intermediate compartment, and Golgi apparatus. J Cell Biol 126:41-52 (1994). Ou WJ et al. Association of folding intermediates of glycoproteins with calnexin during protein maturation. Nature 364:771-6 (1993). Wada I et al. SSR alpha and associated calnexin are major calcium binding proteins of the endoplasmic reticulum membrane. J Biol Chem 266:19599-610 (1991).