Description:
Cellular Localization: Endoplasmic reticulum
Positive Control: Heat Shocked HeLa Cell Lysate.
Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation and Immunocytochemistry. Other applications not tested.
Recommended Dilution:
Flow Cytometry: 1:100
Immunocytochemistry: 1:200
Immunoprecipitation: 1:100
Western Blot” 1:2000
Optimal dilutions to be determined by the researcher.
Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, aliquot Store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
|
| Type | Isotype | Clone | Grade |
|---|
| Pab | IgG | | Serum |
|
| Size | | Storage | Shipping | Source | Host |
|---|
| 100ul | | -20°C | Blue Ice | Human | Rabbit |
|
| Concentration: |
| Not determined |
| Immunogen: |
| Synthetic peptide: DTSAPTSPKVTYKAPVPSG conjugated to KLH, corresponding to amino acids 50-68 of Human Calnexin. |
| Purity: |
| Serum |
| Form |
| Whole Serum. No preservative |
| Specificity: |
| Species Crossreactivity: Crossreacts with Rat, Xenopus laevis, bovine, Chicken, Drosophilia melanogaster, Guinea pig, Hamster, Pig, Quail, Rabbit and Sheep. Expected to crossreact with Human (100% identity with immunogen), Mouse (100% identity with immunogen) and canine (100% identity with immunogen) due to sequence homology. |
|
| Intended for research use only. Not for use in human, therapeutic, or diagnostic applications. |
Hammond C & Helenius A Quality control in the secretory pathway: retention of a misfolded viral membrane glycoprotein involves cycling between the ER, intermediate compartment, and Golgi apparatus. J Cell Biol 126:41-52 (1994). Ou WJ et al. Association of folding intermediates of glycoproteins with calnexin during protein maturation. Nature 364:771-6 (1993). Wada I et al. SSR alpha and associated calnexin are major calcium binding proteins of the endoplasmic reticulum membrane. J Biol Chem 266:19599-610 (1991).
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