Technical Data
Calnexin - ER Marker
Calnexin is a calcium-binding protein which interacts with newly synthesized glycoproteins in ER for facilitating protein assembly and/or the retention within ER of unassembled protein subunits as well as incorrectly folded proteins thereby playing a key role in ER's quality control apparatus. The specificity of calnexin for glycoproteins is defined by a lectin site, which binds an early oligosaccharide intermediate on the folding glycoprotein. When associated with partial T-cell antigen receptor complexes which escape immature thymocytes's ER, calnexin regulates thymocyte maturation process and is also implicated in receptor-mediated endocytosis at synapses. MAPK3/ERK1 phosphorylates calnexin at Ser-565 leading to increased calnexin-ribosomal association and DHHC6 mediated calnexin palmitoylation leads to its preferential localization to perinuclear rough ER. Palmitoylation also facilitate calnexin association with RTC/ribosome-translocon complex that is essential to efficient folding of glycosylated proteins.

Suitable for use in Immunocytochemistry/Immunofluorescence, Western Blot, Immunoprecipitation and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunocytochemistry/Immunofluorescence: 1:100
Western Blot: 1:1000
Immunoprecipitation: 1:100
Immunohistochemistry (Paraffin): 1:100, using antigen retrieval with sodium citrate buffer (pH 6.0).
Optimal dilutions to be determined by the researcher.

Positive Control:
HeLa cells, HeLa whole cell lysate, Mouse bladder tissue.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
A synthetic peptide corresponding to a portion of the C-terminal between aa550-591 of human Calnexin protein (P35565). Species Sequence Homology: Xenopus, canine, orangutan, rat, bovine, porcine, chicken, Drosophilia, guinea pig, quail, rabbit, sheep 100%
Purified by Protein A affinity chromatography.
Supplied as a liquid (serum) in PBS, containing 50% glycerol aand sodium azide.
Recognizes human Calnexin at ~90 kD (actual weight: 97kD). Species Crossreactivity: mouse. Cellular Localization: Endoplasmic reticulum.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Hammond C & Helenius A Quality control in the secretory pathway: retention of a misfolded viral membrane glycoprotein involves cycling between the ER, intermediate compartment, and Golgi apparatus. J Cell Biol 126:41-52 (1994). Ou WJ et al. Association of folding intermediates of glycoproteins with calnexin during protein maturation. Nature 364:771-6 (1993). Wada I et al. SSR alpha and associated calnexin are major calcium binding proteins of the endoplasmic reticulum membrane. J Biol Chem 266:19599-610 (1991).