Technical Data
C1035-26J4
Calnexin, CT (CANX, FLJ26570, IP90, Major histocompatibility complex class I antigen-binding protein p88, p90, P90)
Description:
Calnexin, an abundant ~90kDa integral protein of the endoplasmic reticulum, is also referred to as IP90, p88 and p90 (1). It consists of a large 50kDa N-terminal calcium-binding luminal domain, a single transmembrane helix and a short acidic cytoplasmic tail (2, 3). Unlike its
ER counterparts which have a KDEL sequence on their C-terminus to ensure ER retention (4), calnexin has positively charged cytosolic residues that do the same thing (3). Most ER proteins act as molecular chaperones and participate in the proper folding of polypeptides and their assembly into mulitsubunit proteins. Calnexin together with calreticulin, plays a key role in glycoprotein folding and its control within the ER, by interacting with folding intermediates via their monoglycosylated glycans (5, 6). Calnexin has also been shown to associate with the major histocompatability complex class I heavy chains, partial complexes of the T cell receptor and B cell membrane immunoglobulin (7).

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunoprecipitation, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Western Blot:1:2000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
50ug-20CBlue IceCanineRabbit
Concentration:
As reported
Immunogen:
Canine calnexin C-terminal synthetic peptide conjugated to KLH. Identical to human, mouse and rat calnexin sequences over these residues.
Purity:
Purified by Protein A affinity chromatography.
Form
Supplied as a liquid in PBS, pH7.2, 0.09% sodium azide and 50% glycerol.
Specificity:
Recognizes canine calnexin. Species Crossreactivity: human, monkey, mouse, rat, bovine, chicken (weak), guinea pig, hamster, porcine, quail, rabbit, sheep, drosophila (weak), xenopus (weak).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Rajagopalan S., Xu Y., and Brenner M.B. (1994) Science 263(5145):387-90. 2. Tjoelker L.W., et al. (1994) Biochemistry 33:3229. 3. Schrag J. et al. (2001) Molecular Cell 8(3):633-644. 4. Janiszewski M. (2005) J. Biol Chem. 280(49):40813-40819. 5. Elagoz A., Callejo M., Armstrong J., and Rokeach L. A.
(1999) J. Cell Sci. 112:4449-4460. 6. Otteken A. and Moss B. (1996) J Bio Chem. 271(1):97-103.
7. Galvin K. et al. (1992) Proc Natl Acad Sci USA. 89(18):8452-6.