Technical Data
C1035-27H
Calpain 1, Small Subunit (Calcium-activated Neutral Protease, Calcium-activated Neutral Proteinase 1, CANP, CANP I, CANP1, Capa-1, Capa1, Capn1,mu-Calpain, muCANP, muCL, u-Calpain)
Description:
Calcium-dependent cysteine proteinases, or calpains, are widely distributed in mammalian cells. There are 2 distinct molecular forms, calpains 1 and 2, which differ in the quantity of calcium required.Both calpains 1 and 2 are heterodimeric; each is composed of 1 heavy (about 80 kD), CAPN1 and CAPN2, respectively, and 1 shared light (about 30 kD) subunit, CAPNS1. The heavy subunit has a catalytic function, and the light subunit is regulatory.

Applications:
Suitable for use in Flow Cytometry, Western Blot, Immunohistochemistry and Immunocytochemistry. Other applications not tested.

Recommended Dilution:
Flow Cytometry (human/mouse): 1:100
Western Blot (mouse/rat): 1:1000-1:2000
Immunohistochemistry (human/mouse): 1:100-1:250
Immunocytochemistry (human/mouse): 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
MabIgG9H309Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues in human Calpain 1.
Purity:
Supernatant
Form
Supplied as a liquid in 50mM Tris-Glycine, pH 7.4, 0.15M sodium chloride, 0.01% sodium azide, 0.05% BSA, 40% glycerol.
Specificity:
Recognizes human Calpain 1. Species Crossreactivity: Mouse and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Online Mendelian Inheritance in Man (OMIM), John Hopkins University, Calpain, small subunit 1; CAPNS1 (114170)