Technical Data
Calretinin (Calbindin, CAB29, CAL2)
Calretinin is an intracellular calcium-binding protein belonging to the troponin C superfamily characterized by a structural motif described as the EF-hand domain. The immunohistochemical detection of calretinin in developing cerebellum is restricted to the later stages indicated by weak staining from week 21 of gestation, in Purkinje and basket cells and in neurons of the dentate nucleus. The intensity of staining increases as the cerebellum matures. In tumors, calretinin has been detected in mesotheliomas and some pulmonary adenocarcinomas.

Suitable for use in Immunohistochemistry. Not recommended for Western Blot. Other applications not tested.

Recommended Dilutions:
Immunohistochemistry (Formalin/paraffin): 1:40-1:80 for 60 minutes at RT. Staining of formalin-fixed tissues requires boiling tissue sections in 10mM citrate buffer, pH 6.0 for 10-20 minutes, followed by cooling at RT for 20 minutes.
Optimal dilutions to be determined by the researcher.

Positive Control:

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ul-20CBlue IceMouseMouse
Not Determined
Recombinant, full length mouse calretinin protein. MW of Antigen: 31.5kD. Cellular Localization: Cytoplasmic.
Tissue culture supernatant
Supplied as a liquid, 0.09% sodium azide.
Recognizes mouse Calretinin. Species Crossreactivity: human and rat.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yew D.T., et al., (1997) Developmental Brain Research. 103:37-45. 2. Doglioni, C., et al., (1996) Am. J. Surg. Pathol. 20(9):11037-1046. 3. Parmentier M, et al., (1991) Eur. J. Biochem. 196:79-85.