Technical Data
C1036-77
CaM Kinase II, alpha, phosphorylated (Thr286) (Calmodulin-Dependent Protein Kinase II)
Description:
Applications:
Suitable for use in ELISA and Immunoblot. Other applications not tested.

Recommended Dilutions:
Immunoblot: 0.5-2.0ug/ml
Optimal dilutions to be determined by the researcher.

Immunoblot Analysis:
2ug/ml detected phosphorylated CaM kinase II a subunit, Mr ~50kD, in a rat brain microsomal protein preparation. Pretreating blots with lambda phosphatase (400 units/ml), prior to incubation reduced the signal strength. Treatment with lambda phosphatase had no effect on the signal strength using a non-phosphospeci c CaM Kinase II antibody. A rat brain microsomal preparation was resolved by electrophoresis, transferred to nitrocellulose and incubated without lambda phosphatase or incubated with lambda phosphatase before being probed with anti-phospho-CaM Kinase II (2ug/ml). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and a chemiluminescence detection system.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage, store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG13i29Affinity Purified
SizeStorageShippingSourceHost
100ug4°C (-20°C Glycerol)Blue IceRatMouse
Concentration:
~1mg/ml
Immunogen:
KLH-conjugated, synthetic peptide corresponding to amino acids 281–294 (MHRQE[pT]-VDCLKKFN) of rat Cam Kinase II a-subunit.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as liquid in 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30%.
Specificity:
Recognizes phospho-CaM Kinase II a subunit, Mr 50kD. Additional proteins observed at 125kD and 135kD. Species crossreactivity: Rat. Predicted to react with mouse, human and chicken based on sequence homology.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Patton, B.L., et al., Mol. Biol. Cell. 4: 159–172, 1993.