Technical Data
CaM Kinase IV (Calmodulin-Dependent Protein Kinase IV, CAMKIV)
CaM kinase IV (CaMKIV), in a calcium and calmodulin dependent manner, is implicated in phosphorylating numerous transcription factors including the CCAAT enhancer binding protein (C / EBP), serum response factor (SRF), and cyclic AMP response element binding protein (CBP). The nuclear localization of this protein is consistent with its role in mediating calcium-dependent gene expression. CaMKIV is particularly abundant in testis, T-cells, and neurons but is also found in other tissues to varying degrees. In neurons, CaMKIV is thought to play an important role in synaptic plasticity via its gene regulatory effects. In T-cells, this protein plays an important role in calcium signaling which could affect the transcription regulatory protein, nuclear factor of activated T-cells (NFAT). CaMKIV is encoded, along with calspermin, by the CaMKIV gene. It has been found that, in testes, CaMKIV is expressed in germ cells and found to be associated with chromatin. The association of CaMKIV with chromatin suggests a potential role in chromatin remodeling during nuclear condensation in spermatids.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Western Blot: 0.5ug/ml detects an ~55kD protein representing CaMKIV in Hela cell lysate.
Immunohistochemistry (frozen): 1ug/ml
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceMouseRabbit
Synthetic peptide corresponding to aa127-143, V(127)EKGYYSERDAADAVKQ(143)C from mouse CaMKIV. This sequence is 94% conserved in rat and human CaMKIV.
Purified by immunoaffinity chromatography.
PBS, 1 mg/ml BSA and 0.05% sodium azide
Recognizes mouse Cam Kinase IV at ~55kD. Species Crossreactivity: human, rat, canine, primate, arabidopsis, Xenopus oocyte samples and plant.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
Krebs J Calmodulin-dependent protein kinase IV: regulation of function and expression. Biochim Biophys Acta 1448:183-9 (1998).