Technical Data
Media
Campy Selective Agar Base (Powder)
C1038
Microbiological Grade
Storage RT/4C    Shipping RT
Formulation per g/Liter
Components:
Enzymatic Digest of Animal Tissue10g
Enzymatic Digest of Casein10g
Sodium chloride5g
Agar12g
Total:37g
Antimicrobials / 10ml
Polymyxin B 5000IU
Trimethoprim 10mg
Rifampin 10mg
Cycloheximide 100mg
Campy Selective Agar Base (Powder) is highly nutritive medium for the selective isolation of Campylobacter jejuni and Campylobacter coli. Many experts consider Campylobacter to be the leading cause of enteric illness in the US (1). Campylobacter spp. pathogens are highly infective and transmitted by contaminated food or water. Campy Selective Agar Base (Powder) is based on the formulation described by Bolton and Robertson (2). This formula was developed to isolate Campylobacter spp. from human, animal and environmental specimens. The Preston formulation demonstrated improved recovery and selectivity of Campylobacter spp. in comparative studies with other selective media (Skirrow, Butzler, Blaser and Campy-Blood Agar). Enzymatic Digest of Animal Tissue and Enzymatic Digest of Casein are the nitrogen and vitamin source in this medium. Sodium chloride provides the osmotic environment. Agar is the solidifying agent. Antimicrobics are added to suppress normal enteric flora, and enhance the growth of Campylobacter spp. The addition of 5% lysed horse blood provides essential growth factors.

Quality Control Cultural Response:
Medium was prepared according to label directions and inoculated with the organisms listed below. Campylobacter sp. test strains were incubated at 42 1C in a microaerophilic atmosphere (consisting of ~5-6% oxygen, 3-10% carbon dioxide, 84-85% nitrogen). All other test strains were incubated at 42 1C in an aerobic atmosphere.

Campylobacter coli (ATCC 33559):
Growth (Innoculum: 10-300cfu): Fair to excellent recovery

Campylobacter jejuni (ATCC 29428):
Growth (Innoculum: 10-300cfu): Fair to excellent recovery

Campylobacter jejuni (ATCC 33291):
Growth (Innoculum: 10-300cfu): Fair to excellent recovery

Candida albicans (ATCC 10231):
Growth (Innoculum: ~1000cfu): Markedly suppressed

Enterococcus faecalis (ATCC 29212):
Growth (Innoculum: ~1000cfu): Inhibited

Proteus mirabilis (ATCC 12453):
Growth (Innoculum: ~1000cfu): Inhibited

Notes:
Campylobacter colonies are round to irregular with smooth edges. They may have translucent, white colonies to spreading, flat, transparent growth. Some strains appear tan or slightly pink. Normal enteric flora are completely to markedly inhibited. Typically, Campylobacter spp. are oxidase positive and catalase positive. For complete identification of species and biotype, refer to the appropriate procedures for biochemical reactions (1,4). Due to nutritional variation, some strains may be encountered that grow poorly or fail to grow on this medium.

The antibiotic supplement is not necessary for the recovery of pure isolates of Campylobacter. It is necessary for recovering Campylobacter from mixed flora. This supplement is for the selective isolation of C. jejuni and C. coli. The C. fetus species may not be recovered on C1038 when supplemented with these antiboiotics. Yeasts, gram positive and gram negative "nuisance" strains will be inhibited when the medium is supplemented with these antiboiotics.


Directions per Liter: Dissolve 37 grams per liter of distilled/deionized (DDI) water. Heat with stirring. Boil 1 minute until completely solubilized. Adjust pH as necessary. Dispense into appropriate containers. Loosen caps or cover with foil. Autoclave for 15 minutes at 121C (15psi). Aseptically add 5% lysed horse blood and 10ml of filter sterilized aqueous solution of antimicrobials.

Storage and Stability: Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4C and used within a short period of time.

Appearance: Light beige, homogeneous, free flowing powder. Solubility


Important Note: This product as supplied is intended for research use only, not for use in human, therapeutic or diagnostic applications without the expressed written authorization of United States Biological.

1. U.S. Food and Drug Administration. Bacteriological analytical manual, 8th ed. (1995). AOAC International, Gaithersburg, MD. 2. Bolton, F.J., Robertson, L..,. J. Clin. Microbiol. 35: 462-467 (1982). 3. Bolton, F.J., Coates, D., Hinchliffe, P.M., Robertson, L., J. Clin. Pathol. 36: 78-83 (1983). 4. Murray, P.R., Baron, E.J., Pfaller, M.A., Tenover, F.C., Yolken, R.H. (eds.). Manual of clinical microbiology, 6th ed. (1995). American Society for Microbiology, Washington, D.C.