Technical Data
C1075-98B
Cap-G (Condensin Complex Subunit 3, Chromosome-associated Protein G, Condensin Subunit CAP-G, hCAP-G, Melanoma Antigen NY-MEL-3, Non-SMC Condensin I Complex Subunit G, XCAP-G Homolog, NCAPG, CAPG, NYMEL3)
Description:
Capping protein (actin filament), gelsolin like protein, first purified from rabbit alveolar macrophages, was named macrophage capping protein. The protein was subsequently renamed Cap G (gene symbol CAPG). 'Cap' was chosen because unlike other members of the gelsolin/villin family, this protein caps the barbed ends of actin filaments but does not sever them. 'G' was included in the name because the derived amino acid sequence of Cap G most closely resembles gelsolin, possessing 49% identity with its amino terminal half.

Applications:
Suitable for use in ELISA. Other applications not tested.

Recommended Dilution:
ELISA: 1:100-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for at least 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined.
Immunogen:
Synthetic peptide corresponding to aa251-264 (QQGLNDRSDAVKQA) of human Cap-G.
Purity:
Serum.
Form
Supplied as a liquid, 0.025% sodium azide, 50% glycerol.
Specificity:
Recognizes human Cap-G. Species sequence homology: mouse, rat, chimpanzee, xenopus, chicken.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Strausberg,R.L., Feingold,E.A., et al. Generation and initial analysis of more than 15,000 full-length human and mouse cDNA sequences Proc. Natl. Acad. Sci. U.S.A. 99 (26), 16899-16903 (2002) 2. Walter Witke1, Wei Li2, David J. Kwiatkowski1 and Frederick S. Southwick2 , Comparisons of CapG and gelsolin-null macrophages : demonstration of a unique role for CapG in receptor-mediated ruffling, phagocytosis, and vesicle rocketing, The Journal of Cell Biology, Volume 154, Number 4, August 20, 2001 775-784.