Technical Data
Carbonic Anhydrase IX (CA9)
Carbonic anhydrase (CA) is an enzyme that assists rapid interconversion of carbon dioxide and water into carbonic acid, protons, and bicarbonate ions. It is abundant in all mammalian tissues. Because of its functionality, it has become an important diagnostic marker for various cancers, most notably renal cell carcinoma (RCC). There are many genes that are inducible by hypoxia, via HIF-1 alpha. CA IX is one of the most inducible genes because of its stability and location within the membrane. Carbonic anhydrases have a widespread role in regulating pH in normal tissues, by regulating hydrogen ion (H+) flux. The pH is important in cell death under hypoxia, thus a blockade of CA IX results in increased cell death under hypoxia. Therefore, CA IX has become a reliable histochemical marker of hypoxia.

Suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilutions:
Immunofluorescence: 1:1000
Western Blot: 1:2000
Immunohistochemistry (Frozen): 1:1000. Can also be used with paraffin sections.
Optimal dilutions to be determined by the researcher.

Positive Control:
Renal carcinoma

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months after receipt. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ul-20CBlue IceHumanRabbit
Synthetic peptide corresponding to a C-terminal sequence of the human carbonic anhydrase IX. Cellular Localization: Cell membranes
Purified by immunoaffinity chromatography.
Supplied as a liquid in Tris-citrate/phophate, pH 7.5, 0.09% sodium azide.
Recognizes human carbonic anhydrase IX. Species Crossreactivity: canine, mouse and rat
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Bui, M.H., et al., Clin. Can. Res. 9(2): 802-811 (2003).