Technical Data
C1204-07
Carboxypeptidase A2
Description:
Carboxypeptidase A2 (CPA2) cleaves C-terminal residues with aromatic side chains
including Trp, Tyr and Phe. The 411 aa rhCPA2 consists of a pro region (aa 17 to 112) and
a mature chain (aa 113 to 417).

Applications:
Suitable for use in Western blot, Neutralization, Direct ELISA and Immunoprecipitation. Other applications have not been tested.

Recommended Dilutions:
Western blot:1-2ug/ml the detection limit is~10 ng/lane under non-reducing and reducing conditions. will increase sensitivity by 5 to 50 fold.
Neutralization: preincubate with the activated enzyme at different molar ratios at room temperature for 30 min. Then assay with N-acetyl-Phe-Thiaphe-OH. 50% of the enzymatic activity was inhibited at~ 1.5 ug/ml (IC50) under conditions in which the enzyme was
present at 0.1 ug/ml and the substrate concentration was 0.1 mM. Considering the
molecular masses of the enzyme (42kD) and the antibody (150kD), IC50 was achieved at
approximately 5:1 molar ratio of the antibody to the enzyme.
Immunoprecipitation: 25 ug/ml
Direct ELISA: 0.5-1.0 ug/mL
Optimal dilutions to be determined bythe researcher.

Storage and Stability:

Lyophilized powder may be stored at -20°C. Reconstitute to nominal volume by adding sterile PBS. Aliquot and store at -20°C. Reconstituted product is stable for 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b8B4Affinity Purified
SizeStorageShippingSourceHost
100ug-20°CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
NS0-derived recombinant human rhCPA2.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder from PBS with 5% trehalose . Reconstitute with 1ml sterile PBS.
Specificity:
Recognizes the pro form of human CPA2, but not the activated enzyme in Western blots. Species sequence homology: chimpanzee 99.5%, canine 90%, rat 87%, mouse 86% and chicken 70%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.