Technical Data
C1204-08
Carboxypeptidase A2
Description:
Carboxypeptidase A2 (CPA2) is a pancreatic peptidase that cleaves C-terminal residues with aromatic side chains including Trp, Tyr and Phe. The 411 aa human CPA2 consists of a pro region (aa 17 to 112) and a mature chain (aa 113 to 417).

Applications:
Suitable for use in Western blot, Immunoprecipitation, and Direct ELISA. Other applications have not been tested.

Recommended Dilutions:
Western blot: 1-2ug/ml Using a colorimetric detection system, the detection limit for rhCPA2 is approximately 5 ng/lane under non-reducing and reducing conditions. Chemiluminescent detection with WesternGloTM Chemiluminescent Detection Substrate will increase sensitivity by 5 to 50 fold.
Immunoprecipitation: 25ug/ml
Direct ELISA: 0.5-1.0ug/ml
Optimal dilutions to be determined by the researcher.

Hybridoma:
NSO myeloma cells with spleen cells from Balb/c mice.

Endotoxin: < 0.1 EU per 1ug of the antibody as determined by the LAL method.

Storage and Stability:

Lyophilized powder may be stored at -20C. Reconstitute to nominal volume by adding sterile buffer. Aliquot and store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
MabIgG2b8B5Affinity Purified
SizeStorageShippingSourceHost
100ug-20CBlue IceHumanMouse
Concentration:
~0.5mg/ml
Immunogen:
NS0-derived recombinant human rhCPA2.
Purity:
Purified by Protein G affinity chromatography.
Form
Supplied as a lyophilized powder in PBS with 5% trehalose. Reconstitute with sterile PBS.
Specificity:
Recognizes human CPA2. Recognizes the pro form, (aa 17 - 417) but not the activated enzyme (aa 113 - 417) in Western blots. Does not cross-react with rhCPA1, rhCPA4, rhCPB1 or rhCPE. Species sequence homology: chimpanzee 99.5%,, canine 90%, rat, 87%, mouse 86% and chicken 70%.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.