Technical Data
C1209-06
Carboxypeptidase B (Biotin)
Description:
A carboxypeptidase (EC number 3.4.16 - 3.4.18) is a protease enzyme that hydrolyzes (cleaves) a peptide bond at the carboxy-terminal (C-terminal) end of a protein or peptide. (Contrast with an aminopeptidase, which cleaves peptide bonds at the other end of the protein.) Humans, animals, and plants contain several types of carboxypeptidases that have diverse functions ranging from catabolism to protein maturation. Carboxypeptidase B is a carboxypeptidase that preferentially acts upon basic amino acids, such as arginine and lysine. This serum enzyme is also responsible for rapidly metabolizing the C5a protein into C5a des-Arg, with one less amino acid.

Applications:
Suitable for use in ELISA and Western Blot. Other applications not tested.

Recommended Dilutions:
ELISA: 1:4000-1:25,000; Assayed against 1ug of Carboxypeptidase B in a standard capture ELISA using S7973-97A (Streptavidin (HRP)) and A0030 (ABTS) as a substrate for 30 minutes at RT.
Western Blot: 1:500-1:3000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
Lyophilized powder may be stored at -20C. Stable for 12 months at -20C. Reconstitute with sterile ddH2O. Aliquot to avoid repeated freezing and thawing. Store at -20C. Reconstituted product is stable for 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGHighly Purified
SizeStorageShippingSourceHost
100ug-20CBlue IcePorcineRabbit
Concentration:
~1mg/ml (after reconstitution)
Immunogen:
Carboxypeptidase B (porcine pancreas)
Purity:
Purified from monospecific antiserum by delipidation, salt fractionation and ion exchange chromatography.
Form
Supplied as a lyophilized powder from PBS, pH 7.2, 10mg/ml BSA (IgG and Protease Free), 0.01% sodium azide. Reconstitute with 1ml sterile ddH2O. Labeled with Biotin.
Specificity:
Recognized porcine Carboxypeptidase B. Assay by immunoelectrophoresis resulted in a single precipitin arc against anti-biotin, anti-rabbit serum, purified and partially purified carboxypeptidase B (porcine pancreas).
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.