Technical Data
C1209-55A
CPN1 (Carboxypeptidase N Catalytic Chain, CPN, Carboxypeptidase N Polypeptide 1, Lysine Carboxypeptidase, Arginine Carboxypeptidase, Kininase-1, Serum Carboxypeptidase N, SCPN, Anaphylatoxin Inactivator, Plasma Carboxypeptidase B)
Description:
Carboxypeptidase N is a plasma metallo-protease that cleaves basicaa from the C terminal of peptides and proteins. CPN1 is important in the regulation of peptides like kinins and anaphylatoxins, and it has also been known as kininase-1 and anaphylatoxin inactivator. It is a tetramer comprised of two identical regulatory subunits and two identical catalytic subunits; CPN1 is the catalytic subunit. Mutations in this protein can be associated with angioedema or chronic urticaria resulting from carboxypeptidase N deficiency (1).
Note: The 48kD form is generated by proteolytic cleavage at the C-terminus. This subunit is cleaved into a peptide of 27kD (2).

Applications:
Suitable for use in Western Blot and Immunocytochemistry. Other applications have not been tested.

Recommended Dilutions:
Western Blot: 1:1000-1:10,000
Immunocytochemistry: 1:100-1:250
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Manufactured incorporating RabMAb® technology under Epitomics US patents, No 5,675,063 and 7,429,487, owned by Abcam.
TypeIsotypeCloneGrade
MabEPR6229Supernatant
SizeStorageShippingSourceHost
100ul-20°CBlue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Synthetic peptide corresponding to residues in human CPN1.
Purity:
Supernatant
Form
Supplied as a liquid in PBS, pH 7.2, 0.05% BSA, 0.01% sodium azide, 50% glycerol.
Specificity:
Recognizes human CPN1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Entrez Gene: gene-centered information at NCBI. Nucleic Acids Res. 2005 Jan 1;33:D54-8. 2. Quagraine et al. Biochem J. 2005 May 15;388(Pt 1):81-91.S