Technical Data
CARD10 (Caspase Recruitment Domain, Bimp1, CARMA3)
Apoptosis is related to many diseases and development. Cell death signals are transduced by death domain (DD), death effector domain (DED), and caspase recruitment domain (CARD) containing molecules. CARD containing proteins include some caspases, Apaf-1, CARD4, IAPs,
RICK, ARC, RAIDD, Bcl-10, and ASC. A novel CARD- containing protein was recently identified and designated CARD10 (1). This protein belongs to the membrane- associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules (2). CARD 10 interacts with Bcl-10, a Bcl protein that promotes apoptosis, caspase-9 maturation and activation of NF- B (1,3). CARD 10 and the
related protein CARMA1 also associate with NEMO, the regulatory subunit of the I K complex (4), demonstrating its importance in the regulation of NF- B transcription factor activation

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 5-10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
EL4 cell lysate

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
As reported
Synthetic peptide CARD10 14aa at the C- terminus of human CARD10 (Genbank accession No. AAK26165).
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS, pH 7.2, 0.02% sodium azide.
Recognizes human CARD10. Species Crossreactivity: mouse
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Wang L, Guo Y, Huang W-J, et al. J. Biol. Chem. 2001; 276: 21405-9. 2. Fanning AS and Anderson JM. Curr. Opin. Cell Biol. 1999; 11: 432-9. 3. Fischer KD, Tedford K, Wirth T. Trends Immunol. 2004; 25: 113-6. 4. Stilo R, Liguoro D, Di Jeso B, et al. J. Biol. Chem. 2004; 279: 34323-31.