Technical Data
C1385-25
CARMA1 (Bimp2, CARD11, Caspase Recruitment Domain-containing Protein 11, CARD-containing MAGUK Protein 1)
Description:
CARMA proteins belong to the membrane-associated guanylate kinase-like (MAGUK) family of proteins that can function as molecular scaffolds that assist assembly of signal transduction molecules (1). CARMA1, CARMA2, and CARMA3 share high degrees of sequence and functional homology, but their tissue-specific distribution suggests that they serve distinct biological functions in different cell types (2-4). Both CARMA1 and CARMA3 associate with NEMO, the regulatory subunit of the IkK complex, thereby regulating activation of the NF-kB transcription factor (5). Also, gene inactivation studies showed a complete block in T and B cell immunity as well as an impaired response to LPS, indicating that CARMA1 is a critical regulator in both the adaptive and innate immune systems (6).

Applications:
Suitable for use in Western Blot, Immunohistochemistry and ELISA. Other applications have not been tested.

Recommended Dilution:
Western Blot: 0.5-2ug/ml
Immunohistochemistry: 10ug/ml
Optimal dilutions to be determined by the researcher.

Positive Control:
Human or mouse thymus lysate.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGAffinity Purified
SizeStorageShippingSourceHost
100ug4C (-20C Glycerol)Blue IceHumanRabbit
Concentration:
~1mg/ml
Immunogen:
Synthetic peptide corresponding to amino acids at the C-terminus of human CARMA1 (Genbank accession No. Q9BXL7)
Purity:
Purified by immunoaffinity chromatography.
Form
Supplied as a liquid in PBS, pH 7.4, 0.02% sodium azide.
Specificity:
Recognizes human CARMA1 at ~125kD. Species Crossreactivity: Mouse.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Fanning, A.S. and Anderson, J.M., Curr. Opin. Cell Biol. 11: 432-439 (1999). 2. Gaide, O., Martinon, F., Michau, O., et al., FEBS Lett. 496: 121-127 (2001). 3. Bertin, J., Wang, L., Guo, Y., et al., J. Biol. Chem. 276: 11,877-11,882 (2001). 4. McAllister-Lucas, L.M., Inohara, N., Lucas, P.C., et al., J. Biol. Chem. 276: 30,589-30,597 (2001). 5. Stilo, R., Liguoro, D., Di Jeso, B., et al., J. Biol. Chem. 279: 34,323-34,331 (2004). 6. Hara, H., Wada, T., Bakal, C., et al., Immunity 18: 763-775 (2003).