Technical Data
CARM1 (Coactivator-Associated Arginine Methyltransferase 1, CARM-1, Histone-arginine Methyltransferase CARM1, Protein Arginine N-methyltransferase 4, PRMT4)
CARM1 identified as a SRC-2/TIF2/GRIP1-binding protein, belongs to a family of arginine-specific protein methyltransferases, which includes at least seven members. CARM1 has been shown to synergistically stimulate transcription by nuclear receptors in combination with the p160 family of coactivators and forms a ternary complex with p300/CBP and SRC-2/TIF2/GRIP1. Recent studies broadened the targets of the transcriptional coactivator function of CARM1: CARM1 coactivates p53-dependent transcription and cooperates with -catenin to enhance transcriptional activation by the lymphoid enhancer factor/T-cell factor (LEF1/TCF4). The protein is also a novel transcriptional coactivator of NF- B and functions as a promoter-specific regulator of NF- B recruitment to chromatin. CARM1 also promotes adipocyte differentiation by coactivating PPARgamma-mediated transcription and thus might be important in energy balance. human CARM1 gene is localized on chromosomal region 19p13.2.

Suitable for use in Western Blot. Other applications not tested.

Recommended Dilution:
Western Blot: 1:500-1:1000
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
PabIgGAffinity Purified
100ug-20CBlue IceHumanRabbit
As reported
Synthetic non-phosphopeptide corresponding to human CARM1.
Purified by immunoaffinity chromatography.
Supplied as a liquid in PBS (without Mg2+ and Ca2+), pH 7.4, 150mM sodium chloride, 0.02% sodium azide, 50% glycerol.
Recognizes human CARM1.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Yadav, N. et al. EMBO. Rep. 9:193-198 (2008). 2. Marcela, C. et al. The EMBO Journal. 24:8596 (2005).