Technical Data
C1386-31N
CARM1 (Coactivator-Associated Arginine Methyltransferase 1, CARM-1, Histone-arginine Methyltransferase CARM1, Protein Arginine N-methyltransferase 4, PRMT4)
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Technical Data |
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C1386-31N |
CARM1 (Coactivator-Associated Arginine Methyltransferase 1, CARM-1, Histone-arginine Methyltransferase CARM1, Protein Arginine N-methyltransferase 4, PRMT4) |
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Description: CARM1 identified as a SRC-2/TIF2/GRIP1-binding protein, belongs to a family of arginine-specific protein methyltransferases, which includes at least seven members. CARM1 has been shown to synergistically stimulate transcription by nuclear receptors in combination with the p160 family of coactivators and forms a ternary complex with p300/CBP and SRC-2/TIF2/GRIP1. Recent studies broadened the targets of the transcriptional coactivator function of CARM1: CARM1 coactivates p53-dependent transcription and cooperates with -catenin to enhance transcriptional activation by the lymphoid enhancer factor/T-cell factor (LEF1/TCF4). The protein is also a novel transcriptional coactivator of NF- B and functions as a promoter-specific regulator of NF- B recruitment to chromatin. CARM1 also promotes adipocyte differentiation by coactivating PPARgamma-mediated transcription and thus might be important in energy balance. human CARM1 gene is localized on chromosomal region 19p13.2. Applications: Suitable for use in Western Blot. Other applications not tested. Recommended Dilution: Western Blot: 1:500-1:1000 Optimal dilutions to be determined by the researcher. Storage and Stability: May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. |
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