Technical Data
C2069-39
Catenin, p120, CT
Description:
Originally characterized as a prominent substrate for Src (14) and receptor protein tyrosine kinases (eg, EGF, PDGF, CSF-1) (6), p120ctn is a novel catenin (10, 13) which binds directly to the juxta- membrane region of classical cadherins (eg, E-cadherin)(3 ,11,13,18). Emerging evidence suggests a prominent role in the regulation of cadherin mediated adhesion (1,4,12). Most cells express multiple isoforms of p120 that arise by alternative splicing (8, 9, 11), some of which can be distinguished using monoclonal antibodies (17). Fibroblasts express mostly type 1 isoforms containing an N-terminal extension of 101 amino acids. Type I isoforms may be important for cell motility and are selectively recognized. Epithelial cells preferentially express shorter isoforms lacking this extension. Heterogeneous or negative p120 staining has been reported in breast (5) and colon carcinomas (7, 16) and decreased p120 expression in bladder tumors correlated with a poor prognosis (15). Loss of p120 or E-cadherin never occurs in epithelial tissues under normal circumstances, and its expression is probably mandatory for normal tissue architecture. Like its cousin b-catenin, p120 may have dual roles in both cell-cell adhesion and in nuclear signaling.

Applications:
Suitable for use in Immunofluorescence (canine), ELISA (immunogen), Western Blot and Immunoprecipitation. Other applications not tested.

Recommended Dilution:
Immunofluorescence: 1:250-1:500
ELISA: 1:1000-1:10,000
Western Blot: 1:1000
Immunoprecipitation (Native): 5-10ul/IP reaction
Optimal dilutions to be determined by the researcher.

Positive Control:
MDCK cells (canine), NIH 3T3 cells (mouse), Va-2 broblasts (human), HeLa cells (human), and rat-2 broblasts.

Storage and Stability:
May be stored at 4C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile 40-50% glycerol, aliquot and store at -20C. Aliquots are stable for at least 12 months at -20C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in buffer.
TypeIsotypeCloneGrade
MabIgG1,k15D2Ascites
SizeStorageShippingSourceHost
100ul4C (-20C Glycerol)Blue IceMouse
Concentration:
Not determined
Immunogen:
Recombinant fusion protein derived from the C-terminus of murine p120 Catenin
Purity:
Ascites
Form
Supplied as a liquid, 0.05% sodium azide.
Specificity:
Recognizes p120 Catenin proteins and can be used to detect isoforms 1-4 of the protein. Reacts with a C-terminal epitope found in all known p120 isoforms. Species Crossreactivity: human, mouse, rat and canine. Reactivity with the chicken protein is weak but detectable.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Aono, S., et al., J. Cell Biol. 145: 551-562 (1999). 2. Daniel, J.M. and Reynolds, A.B., Mol. Cell Biol. 19: 3614-3623 (1999). 3. Daniel, J.M. and Reynolds, A.B., Mol. Cell. Biol. 15: 4819-4824 (1995). 4. Daniel, J.M. and Reynolds, A.B., BioEssays 19: 883-991 (1997). 5. Dillon, D.A., et al., Am. J. Pathol. 152: 75-82 (1998). 6. Downing, J.R. and Reynolds, A.B., Oncogene 6: 607-613 (1991). 7. Gold, J.S., et al., Cancer Letters 132: 192-201 (1998). 8. Keirsebilck, A., et al., Genomics 50: 129-146 (1998). 9. Mo, Y.Y. and Reynolds, A.B., Cancer Res. 56: 2633-2640 (1996). 10. Peifer, M., et al., Cell 76: 789-791 (1994). 11. Reynolds, A.B., et al., Mol. Cell. Biol. 14: 8333-8342 (1994). 12. Reynolds, A.B. and Daniel, J.M., (1997) p. 31-48. In P. Cowin, and M. Klymkowsky (ed.), Cytoskeletal-Membrane Interactions and Signal Transduction, Landes Bioscience, USA. 13. Reynolds, A.B., et al., Oncogene 7: 2439-2445 (1992). 14. Reynolds, A.B., et al., Mol. Cell. Biol. 9: 629-638 (1989). 15. Shimazui, T., et al., Cancer Res. 56: 4154-4158 (1996). 16. Skoudy, A., et al., Int. J. Cancer 68: 14-20 (1996). 17. Wu, J., et al., Hybridoma 17: 175-183 (1998). 18. Yap, A., et al., T J. Cell Biol. 141: 779-789 (1998).