Technical Data
C2069-40
Catenin alpha1 (CTNNA1, Catenin (Cadherin-associated Protein), alpha 1, 102kDa, HGNC:2509, CAP102, FLJ36832, alpha 1 (102kD), alpha-E-Catenin, alpha-Catenin, alphaE-Catenin)
Description:
The catenins are polypeptides that bind to the conserved cytoplasmic tail of cadherins and are required for cadherin function (2). a-Catenin is a 102kD protein that is associated with the cadherin indirectly through its interaction with b-catenin and g-catenin (3). b-Catenin and its homolog plakoglobin associate directly with the cadherin (4). Thus, the cell-cell adherens junction is a structure composed of the transmembrane cadherin, which is associated directly with either b-catenin or plakoglobin, which in turn assoctiates directly with a-catenin. a-Catenin then mediates the interaction between the cadherin-catenin complex and the actin cytoskeleton (5,6). a-Catenin has been characterized as a tumor suppressor, suggesting that it may have a regulatory function in regulation to its structural role (7).

Applications:
Suitable for use in Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation and ELISA. Other applications not tested.

Recommended Dilution:
Immunohistochemistry: 1:250-1:500; Formalin-fixed, paraffin-embedded tissues. Requires pressure cooker antigen retrieval method.
Optimal dilutions to be determined by the researcher.

Storage and Stability:
May be stored at 4°C for short-term only. For long-term storage and to avoid repeated freezing and thawing, add sterile glycerol (40-50%), aliquot and store at -20°C. Aliquots are stable for at least 12 months at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
TypeIsotypeCloneGrade
PabIgGSerum
SizeStorageShippingSourceHost
50ul4°C (-20°C Glycerol)Blue IceHumanRabbit
Concentration:
Not determined
Immunogen:
Recombinant Fusion Protein (amino terminal; 576 amino acids)
Purity:
Serum
Form
Supplied as a liquid. No preservatives added.
Specificity:
Recognizes alpha-catenin at 102kD. Species Crossreactivity: Human, mouse and canine.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Dillon, D., et al., American Journal of Pathology 152(1): 75–81 (1998). 2. Gumbiner, B.M., et al., J. Cell Sci. Suppl. 17: 155–158 (1993). 3. Fouquet, B., et al., Differentiation 51: 187–194 (1992). 4. Butz, S., et al., FEBS Letters 355: 195–200 (1994). 5. Nagafuchi, A., et al., J. Cell Biology 127: 235–245 (1994). 6. Shahidul, I., et al., Journal of Cell Biology 135(6.1): 1643–1654 (1996). 7. Rudiger, M., Bioessays 20(9): 733–740 (1998).