Technical Data
Catenin, alpha (Catenin alpha-1, CTNNA1, Alpha E-catenin, CAP102, Cadherin-associated Protein, FLJ36832, FLJ52416, Renal Carcinoma Antigen NY-REN-13)
E-cadherin is a transmembrane glycoprotein responsible for physical connection of epithelial cells through Ca(2+)-binding regions in its extracellular domain. E-cadherin-mediated cell-cell adhesion is effected by 3 cytoplasmic proteins known as catenins alpha, beta, and gamma. These catenins are thought to work as connectors that anchor the E-cadherin to the cytoskeletal actin bundle through the cadherin cytoplasmic domain. Alpha-catenin, a cadherin-associated protein, links cadherin/beta-catenin and cadherin/plakoglobin complexes to the actin cytoskeleton.Thus it is required for the function of cadherins, cell adhesion molecules.The gene encodes 906 amino acids and is mapped to 5q31. Pathological role of alpha-catenin has been observed in human myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). It is expressed ubiquitously in normal tissues. Alternatively spliced transcript variants encoding two isoforms have been identified.

Suitable for use in Western Blot and Immunohistochemistry. Other applications not tested.

Recommended Dilution:
Immunohistochemistry (paraffin): 1:50-1:100
Western Blot: 1:100-750
Optimal dilutions to be determined by the researcher.

Positive Control:
Human lung cell line

Cellular Localization:
Cytoplasmic and cell membrane

Storage and Stability:
May be stored at 4C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20C. Aliquots are stable for 12 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
500ul-20CBlue IceHumanRabbit
Not determined
Synthetic peptide derived from the C-terminus of human Catenin, alpha protein.
Supplied as a liquid in 0.05% sodium azide.
Recognizes human Alpha-Catenin. Species Crossreactivity: mouse and xenopus.
Intended for research use only. Not for use in human, therapeutic, or diagnostic applications.
1. Koslov et al. J Biol Chem 272 (43): 27301, 1997. 2. Rimm et al. Lab Invest 72 (5): 590, 1995.
3. Hazan et al. J Biol Chem 272 (51): 32448, 1997. 4. Taniguchi et al. Int J Mol Med 16:1003, 2005.